NUCLEAR PLOIDY OF NORMAL AND NEOPLASTIC HEPATOCYTES FROM WOODCHUCK HEPATITIS VIRUS-INFECTED AND UNINFECTED WOODCHUCKS
NUCLEAR PLOIDY OF NORMAL AND NEOPLASTIC HEPATOCYTES FROM WOODCHUCK HEPATITIS VIRUS-INFECTED AND UNINFECTED WOODCHUCKS
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DOI：
10.1016/0270-9139(94)90852-4
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发表时间：
1994-05-01
期刊：
影响因子：
13.5
通讯作者：
GEBHARD, DH
中科院分区：
文献类型：
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作者：
CULLEN, JM;LINZEY, DW;GEBHARD, DH
对成年土拨鼠（人嗜肝DNA病毒诱导的肝细胞癌模型）正常和肿瘤肝细胞核的倍性进行流式细胞术分析。来自土拨鼠肝炎病毒感染或未感染肝脏的非肿瘤性肝脏的所有36个细胞核样本均为二倍体，表明与年龄相关的核多倍化不发生在该物种中，与其他啮齿动物不同。从14只土拨鼠肝炎病毒感染的土拨鼠中获得单个或多个肝肿瘤。对19例肝细胞癌和8例腺瘤进行了检查。在10例肝细胞癌和3例腺瘤样本中检测到非整倍体细胞核。10例非整倍体HCC中7例和3例非整倍体腺瘤的DNA指数相似，范围为1.11 ~ 1.22。19例肝细胞癌中有9例为二倍体，8例肝细胞腺瘤中有5例为二倍体。四个二倍体肝细胞癌的四倍体细胞核的比例增加。非整倍体细胞核的存在与肿瘤的组织学表现或血清γ-谷氨酰转肽酶水平无关。因为没有肝细胞癌转移，异倍体的存在可能与生物学行为无关。我们测定了肝细胞癌和非肿瘤性肝脏中单核和双核肝细胞的比例。在土拨鼠肝炎病毒感染和未感染的非肿瘤肝中，约7%的肝细胞为双核细胞。只有2%的恶性肝细胞是双核的。这项研究的结果表明，非整倍体是一种常见的变化，肝肿瘤土拨鼠肝炎病毒感染土拨鼠。
Flow cytometric analysis of the ploidy of normal and neoplastic hepatocyte nuclei obtained from adult woodchucks, a model of human hepadnavirus-induced hepatocellular carcinoma, was performed. All 36 samples of nuclei from non-neoplastic liver from woodchuck hepatitis virus-infected or uninfected liver were diploid, indicating that age-related nuclear polyploidization does not occur in this species, unlike other rodents. Individual or multiple hepatic neoplasms were obtained from each of 14 woodchuck hepatitis virus-infected woodchucks. Nineteen samples of hepatocellular carcinoma and eight adenomas were examined. Aneuploid nuclei were detected in 10 of the hepatocellular carcinomas and three of the adenoma samples. Similar DNA indexes, ranging from 1.11 to 1.22, were found in 7 of the 10 aneuploid HCCs and all 3 aneuploid adenomas. Nine of the 19 hepatocellular carcinoma samples and 5 of the 8 adenomas were diploid. Four of the diploid hepatocellular carcinomas had increased proportions of tetraploid nuclei. The presence of aneuploid nuclei was not related to histological appearance of the neoplasms or serum gamma-glutamyltranspeptidase levels. Because none of the hepatocellular carcinomas metastasized, the presence of aneuploidy could not be related to biological behavior. We determined the proportion of uninucleate and binucleate hepatocytes in hepatocellular carcinoma and nonneoplastic liver. Approximately 7% of hepatocytes were binucleate in nonneoplastic liver from woodchuck hepatitis virus-infected and uninfected liver. Only 2% of malignant hepatocytes were binucleate. The results of this study indicate that aneuploidy is a common change in hepatic neoplasms from woodchuck hepatitis virus-infected woodchucks.