Identification of 32 full-length NAC transcription factors in ramie (Boehmeria nivea L. Gaud) and characterization of the expression pattern of these genes
Identification of 32 full-length NAC transcription factors in ramie (Boehmeria nivea L. Gaud) and characterization of the expression pattern of these genes
复制标题
苎麻 (Boehmeria nivea L. Gaud) 中 32 个全长 NAC 转录因子的鉴定以及这些基因表达模式的表征
DOI：
10.1007/s00438-014-0842-4
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发表时间：
2014-08-01
影响因子：
3.1
通讯作者：
Tang, Shouwei
中科院分区：
文献类型：
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作者：
Liu, Touming;Zhu, Siyuan;Tang, Shouwei
NAM、ATAF和CUC （NAC）基因是植物特异性转录因子（TFs），在植物生长发育和抗逆性中起关键作用。尽管苎麻是最重要的天然纤维作物之一，但迄今为止，还没有一个苎麻NAC （BnNAC）基因被鉴定出来。为了挖掘BnNAC TFs并确定其潜在功能，我们在之前两项研究中从RNA-seq中组装的两个unigenes de novo库中寻找BnNAC基因，本研究共鉴定出32个全长BnNAC基因。对其他物种中已发表的47个功能已知的NAC蛋白与这32个BnNAC蛋白进行了系统发育分析，结果表明，79个NAC蛋白可划分为8个类群（NAC- i - viii）。在32个BnNAC基因中，24个、2个和1个基因分别在茎木质部、叶和花中表达量较高。另外，干旱、镉胁迫和根病线虫侵染分别调控了14、11和4个BnNAC基因的表达。有趣的是，有5个BnNAC TFs与其他参与调控次生壁合成的物种的NAC TFs具有高度的同源性，并且它们的表达不受干旱和镉胁迫的调节。这些结果提示BnNAC家族可能具有功能多样性。
NAM, ATAF, and CUC (NAC) genes are plant-specific transcription factors (TFs) that play key roles in plant growth, development, and stress tolerance. To date, none of the ramie NAC (BnNAC) genes had been identified, even though ramie is one of the most important natural fiber crops. In order to mine the BnNAC TFs and identify their potential function, the search for BnNAC genes against two pools of unigenes de novo assembled from the RNA-seq in our two previous studies was performed, and a total of 32 full-length BnNAC genes were identified in this study. Forty-seven function-known NAC proteins published in other species, in concert with these 32 BnNAC proteins were subjected to phylogenetic analysis, and the result showed that all the 79 NAC proteins can be divided into eight groups (NAC-I–VIII). Among the 32 BnNAC genes, 24, 2, and 1 gene showed higher expression in stem xylem, leaf, and flower, respectively. Furthermore, the expression of 14, 11 and 4 BnNAC genes was regulated by drought, cadmium stress, and infection by root lesion nematode, respectively. Interestingly, there were five BnNAC TFs which showed high homology with the NAC TFs of other species involved in regulating the secondary wall synthesis, and their expressions were not regulated by drought and cadmium stress. These results suggested that the BnNAC family might have a functional diversity.