Construction of Epstein-Barr virus-based expression vector containing mini-oriP
Construction of Epstein-Barr virus-based expression vector containing mini-oriP
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DOI：
10.1006/bbrc.1999.1617
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发表时间：
1999-11-02
影响因子：
3.1
通讯作者：
Inoue, H
中科院分区：
文献类型：
--
作者：
Tanaka, J;Miwa, Y;Inoue, H
基于 Epstein-Barr 病毒 (EBV) 的载体是携带复制起点 oriP（约 2200 bp）和足以自主复制的复制起始因子 (EBNA-1) 的染色体外载体。由于这些载体的一个缺点是尺寸较大，因此我们使用增强型绿色荧光蛋白 (EGFP) 作为报告基因来监测基因表达，研究了 oriP 的部分缺失对基于 EBV 的载体有效性的影响。结果表明，954 bp 缺失的 mini-oriP 在灵长类细胞中非常有用，因为该载体表现出高效率的稳定转染、高比例的 EGFP 阳性细胞以及从转染细胞中回收的完整质粒 DNA。 (C) 1999 年学术出版社。
Epstein-Barr virus (EBV)-based vectors are extrachromosomal vectors carrying a replicational origin, oriP (about 2200 bp) and a replication initiation factor (EBNA-1) which are sufficient for autonomous replication. Because one disadvantage of these vectors is their large sizes, we examined the effect of partial deletion of oriP on the effectiveness of the EBV-based vectors, using an enhanced green fluorescent protein (EGFP) as a reporter to monitor gene expression. Results indicated that 954 bp-deleted mini-oriP is useful in primate cells since the vector showed high efficiency of stable transfection, a high ratio of EGFP-positive cells, and high recovery of intact plasmid DNA from transfected cells. (C) 1999 Academic Press.