2 TOBACCO DNA-BINDING PROTEINS WITH HOMOLOGY TO THE NUCLEAR FACTOR CREB
2 TOBACCO DNA-BINDING PROTEINS WITH HOMOLOGY TO THE NUCLEAR FACTOR CREB
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DOI：
10.1038/340727a0
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发表时间：
1989-08-31
期刊：
影响因子：
64.8
通讯作者：
CHUA, NH
中科院分区：
文献类型：
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作者：
KATAGIRI, F;LAM, E;CHUA, NH
花椰菜花叶病毒 (CaMV) 的 35S 启动子在 -83 至 -63 区域包含序列 TGACG 的串联重复序列。这个 21 碱基对 (bp) 序列称为 as-1，参与 35S 启动子的根表达。当插入在光合组织中特异性表达的基因的启动子时，as-1 可在根中实现高水平表达13。我们已经描述了一种因子，ASF-1，它在体外特异性结合 toas-1。 ASF-1与as-1相关序列的体外结合亲和力与这些序列的体内功能之间存在良好的相关性。这些结果强烈表明 ASF-1 负责 as-1 的功能（参考文献 13）。在这里，我们报告了编码两种 TGACG 序列特异性结合蛋白（TGAla 和 TGAlb）的烟草互补 DNA 克隆的分离。 cDNA 克隆的序列分析表明，这两种蛋白都含有一个碱性区域，该区域与核因子 CREB、GCN4 和 c-Jun1-4 中的一段碱性氨基酸与“亮氨酸拉链”区域具有高度同源性5。基于结合特异性，我们建议 TGAla 是 ASF-1 的良好候选者。
THE 35S promoter of the cauliflower mosaic virus (CaMV) contains a tandem repeat of the sequence TGACG in the region −83 to −63. This 21-base pair (bp) sequence, calledas-1, is involved in root expression of the 35S promoter. When inserted in a promoter of a gene expressed specifically in photosynthetic tissues,as-1confers high level expression in roots13. We have described a factor, ASF-1, that binds specifically toas-1 in vitro. There is a good correlation between ASF-1 binding affinity toas-1related sequencesin vitroand the function of these sequencesin vivo. These results strongly suggest that ASF-1 is responsible for the function ofas-1(ref. 13). Here we report the isolation of tobacco complementary DNA clones encoding two TGACG-sequence-specific binding-proteins (TGAla and TGAlb). Sequence analysis of the cDNA clones shows that both proteins contain a basic region that shows high homology to a stretch of basic amino acids in the nuclear factors CREB, GCN4, and c-Jun1-4to a 'leucine-zipper' region5. On the basis of binding specificity we propose TGAla to be a good candidate for ASF-1.