Study of host-microbe interactions in zebrafish.
Study of host-microbe interactions in zebrafish.
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DOI：
10.1016/b978-0-12-381320-6.00004-7
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发表时间：
2011
影响因子：
--
通讯作者：
Kim CH
中科院分区：
文献类型：
--
作者：
Milligan-Myhre K;Charette JR;Phennicie RT;Stephens WZ;Rawls JF;Guillemin K;Kim CH
所有动物都是生态系统，是各种微生物种群的家园。动物相关微生物在宿主的正常发育和生理中起着重要作用，但也可能是传染病的病原体。传统上，小鼠被用于研究微生物和脊椎动物之间的致病性和有益性关系。斑马鱼正在成为宿主-微生物相互作用研究的一个有价值的新模型系统，为研究人员提供了在活体动物细胞水平上调查大量宿主和可视化微生物-宿主关联的机会。本章提供了详细的协议，分析斑马鱼相关的微生物群落，无菌斑马鱼的衍生和饲养，并通过不同的接种途径在斑马鱼发育的不同阶段建立传染病模型。这些协议为研究人员提供了一个起点，以解决有关动物与微生物共存的众多问题。
All animals are ecosystems, home to diverse microbial populations. Animal-associated microbes play important roles in the normal development and physiology of their hosts, but can also be agents of infectious disease. Traditionally, mice have been used to study pathogenic and beneficial associations between microbes and vertebrate animals. The zebrafish is emerging as a valuable new model system for host-microbe interaction studies, affording researchers with the opportunity to survey large populations of hosts and to visualize microbe-host associations at a cellular level in living animals. This chapter provides detailed protocols for the analysis of zebrafish-associated microbial communities, the derivation and husbandry of germ-free zebrafish, and the modeling of infectious disease in different stages of zebrafish development via different routes of inoculation. These protocols offer a starting point for researchers to address a multitude of questions about animals’ coexistence with microorganisms.