IMPROVEMENTS OF DNA SEQUENCING GELS
IMPROVEMENTS OF DNA SEQUENCING GELS
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DOI：
10.1016/0003-2697(81)90031-2
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发表时间：
1981-01-01
影响因子：
2.9
通讯作者：
ANSORGE, W
中科院分区：
文献类型：
--
作者：
GAROFF, H;ANSORGE, W
描述了 DNA 测序凝胶的三种简单修改，所有这些修改都导致寡核苷酸分辨率的提高，如放射自显影术所示。通过使用新的凝胶成型技术，可以将凝胶的厚度减少到 0.2 毫米。通过预先将凝胶粘合到玻璃板的表面，可以将凝胶干燥而不会使其尺寸发生任何变形。通过将其中一块玻璃板更换为带有循环水的廉价恒温板，在电泳过程中凝胶的所有部分都获得了均匀的高温。该加热板的使用导致整个凝胶上出现直条带图案，并且还提高了其他电泳系统中无法解决的此类条带的分辨率。
Three simple modifications of DNA sequencing gels are described which all result in improved oligonucleotide resolution as visualized by autoradiography. It was possible to reduce the thickness of the gel to 0.2 mm by using new gel molding techniques. The gel could be dried without any distortions of its dimensions by prior binding of the gel to the surface of the glass plate. A uniform high temperature was obtained in all parts of the gel during electrophoresis by replacing one of the glass plates with an inexpensive thermostating plate with circulating water. The use of this heating plate resulted in a straight band pattern all over the gel and also in the resolution of such bands which were not resolved in other electrophoresis systems.