Galleria mellonella as a model host to study infection by the Francisella tularensis live vaccine strain
Galleria mellonella as a model host to study infection by the Francisella tularensis live vaccine strain
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DOI：
10.1016/j.micinf.2007.02.016
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发表时间：
2007-05-01
影响因子：
5.8
通讯作者：
Mylonakis, Eleftherios
中科院分区：
文献类型：
--
作者：
Aperis, George;Fuchs, Beth Burgwyn;Mylonakis, Eleftherios
利用土拉弗朗西斯菌活疫苗株（Francisella tularensis）对大蜡螟（Galleria mellonella，鳞翅目：螟蛾科;大蜡螟）幼虫的杀灭作用，建立了一个可用于研究土拉弗朗西斯菌的无脊椎动物宿主系统。土拉菌感染和抗菌化合物对F.土拉菌将F. tularensis LVS，杀死毛虫，尽管LVS与血细胞的关联。杀灭率取决于注射的细菌数量。在接种前或接种后给予环丙沙星、左氧氟沙星或链霉素的抗生素治疗可延长存活时间，并降低组织负荷。血腔中的土拉热。延迟药物治疗降低了抗菌药物的疗效，尤其是链霉素。共和党大蜡螟F. tularensis LVS系统的建立有助于F.土拉热，抗菌剂的疗效。(C)2007年，Elsevier Masson SAS。All rights reserved.
We used the killing of Galleria mellonella (Lepidoptera: Pyralidae; the greater wax moth) caterpillar by the live vaccine strain (LVS) of Francisella tularensis to develop an invertebrate host system that can be used to study F. tularensis infection and the in vivo effects of antibacterial compounds on F. tularensis LVS. After injection into the insect hemocoel, F. tularensis LVS, killed caterpillars despite the association of LVS with hemocytes. The rate of killing depended on the number of bacteria injected. Antibiotic therapy with ciprofloxacin, levofloxacin or streptomycin administered before or after inoculation prolonged survival and decreased the tissue burden of F. tularensis in the hemocoel. Delayed drug treatment reduced the efficacy of antibacterials and especially streptomycin. The G. mellonella-F. tularensis LVS system may facilitate the in vivo study of F. tularensis, efficacy with antibacterial agents. (C) 2007 Elsevier Masson SAS. All rights reserved.