The GNU subunit of PNG kinase, the developmental regulator of mRNA translation, binds BIC-C to localize to RNP granules.
The GNU subunit of PNG kinase, the developmental regulator of mRNA translation, binds BIC-C to localize to RNP granules.
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DOI：
10.7554/elife.67294
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发表时间：
2021-07-12
期刊：
影响因子：
7.7
通讯作者：
Orr-Weaver TL
中科院分区：
文献类型：
--
作者：
Avilés-Pagán EE;Hara M;Orr-Weaver TL
mRNA翻译的控制是分化的卵母细胞转变为全能胚胎的关键机制。在果蝇中，PNG激酶复合物在卵母细胞向胚胎转变时调节母体mRNA的翻译。我们先前表明，GNU激活亚基在调节PNG和将其活性定时到卵激活和早期胚胎发生之间的窗口中是至关重要的（Hara等人，2017年）。在这项研究中，我们发现GNU和RNP颗粒蛋白质之间的关联，并证明GNU定位于成熟卵母细胞的细胞质RNP颗粒，确定GNU作为RNP颗粒的一个子集的新组件。此外，我们定义了GNU领域的角色。GNU和颗粒组分BIC-C之间的相互作用揭示了后生动物发育中翻译调控的潜在保守功能。我们提出，通过结合BIC-C，在卵激活后，GNU将PNG带到其最初的目标，RNP颗粒中的翻译阻遏物。
Control of mRNA translation is a key mechanism by which the differentiated oocyte transitions to a totipotent embryo. In Drosophila, the PNG kinase complex regulates maternal mRNA translation at the oocyte-to-embryo transition. We previously showed that the GNU activating subunit is crucial in regulating PNG and timing its activity to the window between egg activation and early embryogenesis (Hara et al., 2017). In this study, we find associations between GNU and proteins of RNP granules and demonstrate that GNU localizes to cytoplasmic RNP granules in the mature oocyte, identifying GNU as a new component of a subset of RNP granules. Furthermore, we define roles for the domains of GNU. Interactions between GNU and the granule component BIC-C reveal potential conserved functions for translational regulation in metazoan development. We propose that by binding to BIC-C, upon egg activation GNU brings PNG to its initial targets, translational repressors in RNP granules.