Perspectives on Mucus Properties and Formation-Lessons from the Biochemical World
Perspectives on Mucus Properties and Formation-Lessons from the Biochemical World
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DOI：
10.1101/cshperspect.a014159
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发表时间：
2012-11-01
影响因子：
5.4
通讯作者：
Hansson, Gunnar C.
中科院分区：
文献类型：
--
作者：
Ambort, Daniel;Johansson, Malin E. V.;Hansson, Gunnar C.
我们的MUC2粘蛋白模型显示了一个组织良好的网状凝胶，由六个不同的共价键和非共价键交联。当MUC2粘蛋白被包装在粘蛋白颗粒中时，它由在高钙和低pH下形成的氨基末端连接环平台组织。这种包装允许有序释放和正常的粘蛋白膨胀，当钙被去除并且通过碳酸氢盐增加pH时。在缺乏囊性纤维化跨膜传导调节因子（CFTR）依赖性碳酸氢盐转运的情况下，该过程是有缺陷的。扩展的分泌粘蛋白被认为是通过MUC 2粘蛋白中遗传的特性和蛋白水解过程而自组织的。
Our model of the MUC2 mucin shows a well-organized netlike gel that is cross-linked by six different covalent and noncovalent bonds. When the MUC2 mucin is packed in the mucin granule it is organized by an amino-terminal concatenated ring platform formed at high calcium and low pH. This packing allows an ordered release and a normal mucin expansion when calcium is removed and pH increased by bicarbonate. This process is defective in the absence of cystic fibrosis transmembrane conductance regulator (CFTR)-dependent bicarbonate transport. The expanded secreted mucin is suggested to be self-organizing by properties inherited in the MUC2 mucin and by proteolytic processes.