Graphene Induces Formation of Pores That Kill Spherical and Rod-Shaped Bacteria
Graphene Induces Formation of Pores That Kill Spherical and Rod-Shaped Bacteria
复制标题
DOI：
10.1021/acsnano.5b03368
复制
发表时间：
2015-08-01
期刊：
影响因子：
17.1
通讯作者：
Ivanova, Elena P.
中科院分区：
文献类型：
--
作者：
Pham, Vy T. H.;Vi Khanh Truong;Ivanova, Elena P.
原始石墨烯、其衍生物和复合材料已被广泛报道具有抗菌性能。大多数模拟细菌细胞膜和石墨烯表面之间相互作用的研究都提出，石墨烯诱导的细菌细胞死亡是由（1）插入刀片状石墨烯基纳米片或（2）通过亲脂性石墨烯的存在破坏性提取脂质分子引起的。然而，这些模拟研究仅考虑石墨烯-细胞膜相互作用，其中石墨烯处于分散形式。在本文中，我们报告了石墨烯片表面的抗菌行为，试图进一步推进目前的知识，石墨烯细胞毒性使用实验和计算机模拟方法。石墨烯纳米膜被制造成在石墨烯片中表现出不同的边长和不同的取向角。将这些底物与铜绿假单胞菌和金黄色葡萄球菌接触，发现这些底物对这两种病原菌表现出可变的杀菌效率。结果表明，石墨烯的边缘的密度是有助于石墨烯纳米片膜的抗菌行为的主要参数之一。该研究提供了实验和理论证据，证明石墨烯纳米片的抗菌行为源于细菌细胞壁中孔的形成，导致随后的渗透失衡和细胞死亡。
Pristine graphene, its derivatives, and composites have been widely reported to possess antibacterial properties. Most of the studies simulating the interaction between bacterial cell membranes and the surface of graphene have proposed that the graphene-induced bacterial cell death is caused either by (1) the insertion of blade-like graphene-based nanosheets or (2) the destructive extraction of lipid molecules by the presence of the lipophilic graphene. These simulation studies have, however, only take into account graphene-cell membrane interactions where the graphene is in a dispersed form. In this paper, we report the antimicrobial behavior of graphene sheet surfaces in an attempt to further advance the current knowledge pertaining to graphene cytotoxicity using both experimental and computer simulation approaches. Graphene nanofilms were fabricated to exhibit different edge lengths and different angles of orientation in the graphene sheets. These substrates were placed in contact with Pseudomonas aeruginosa and Staphylococcus aureus bacteria, where it was seen that these substrates exhibited variable bactericidal efficiency toward these two pathogenic bacteria. It was demonstrated that the density of the edges of the graphene was one of the principal parameters that contributed to the antibacterial behavior of the graphene nanosheet films. The study provides both experimental and theoretical evidence that the antibacterial behavior of graphene nanosheets arises from the formation of pores in the bacterial cell wall, causing a subsequent osmotic imbalance and cell death.