Colonic expression of heme oxygenase-1 is associated with a better long-term survival in patients with colorectal cancer
Colonic expression of heme oxygenase-1 is associated with a better long-term survival in patients with colorectal cancer
复制标题
DOI：
10.1080/00365520701192383
复制
发表时间：
2007-01-01
影响因子：
1.9
通讯作者：
Fujimori, Takahiro
中科院分区：
文献类型：
--
作者：
Becker, Jan C.;Fukui, Hirokazu;Fujimori, Takahiro
Objective.血红素氧合酶-1（HO-1）已成为胃肠道粘膜防御的重要介质。其代谢途径产物胆绿素/胆红素和一氧化碳可以减少氧化应激和炎症，并促进对细胞凋亡的抵抗。然而，HO-1在胃肠道恶性肿瘤中的作用仍有待阐明。本研究的目的是分析HO-1在人结肠腺瘤和结肠癌样品中的表达。材料和方法。采用抗HO-1单克隆抗体对55例结直肠癌石蜡包埋手术标本和19例结肠腺瘤标本进行HO-1表达的免疫组化染色。HO-1表达由两名不同的研究者独立评估，随后与临床数据和患者的预期寿命相关。结果41.8%（23/ 55）的结直肠癌灶性表达HO-1。HO-1在结肠腺瘤中的表达率为36.8%（7/ 19）。在表达HO-1的结直肠癌样品中，淋巴肿瘤侵袭率显著较低（p = 0.048）。此外，在HO-1表达的结直肠癌样本中发现较少的淋巴结转移，但这些差异未达到统计学显著性。平均观察期为65.87 ± 3.96个月。Kaplan-Meier分析显示结肠HO-1表达的结直肠癌患者的生存率显著更高（p = 0.018）。结论.这项研究表明，结肠HO-1可能是结直肠癌预后的一个标志物。
Objective. Heme oxygenase-1 (HO-1) has emerged as a crucial mediator of mucosal defense in the gastrointestinal tract. Its metabolic pathway products, biliverdin/ bilirubin and carbon monoxide, can reduce oxidative stress and inflammation, and promote resistance to apoptosis. The role of HO-1 in gastrointestinal malignancies, however, remains to be elucidated. The purpose of this study was to analyze HO-1 expression in human colon adenoma and cancer samples. Material and methods. Fifty-five paraffin-embedded surgical specimens of colorectal cancer and 19 colonic adenoma samples were stained immunhistochemically for HO-1 expression using an anti-HO-1 monoclonal antibody. HO-1 expression was evaluated independently by two different investigators and subsequently correlated to clinical data and patients' life expectancy. Results. Focal HO-1 expression could be documented in 41.8% (23/ 55) of patients with colorectal cancer. HO-1 expression in colonic adenoma was detectable in 36.8% (7/ 19) of cases. The rate of lymphatic tumor invasion was significantly lower in colorectal cancer samples expressing HO-1 (p = 0.048). Additionally, fewer lymph node metastases were found in colorectal cancer samples with HO-1 expression, but these differences did not reach statistical significance. Mean observation period was 65.87 +/- 3.96 months. Kaplan-Meier analysis showed a significantly better survival for colorectal cancer patients with colonic HO-1 expression (p = 0.018). Conclusions. This study demonstrates that colonic HO-1 may be a prognostic marker of colorectal-cancer outcome.