MAPPING OF A DOMINANT IMMUNOGENIC REGION OF SYNAPTOPHYSIN, A MAJOR MEMBRANE-PROTEIN OF SYNAPTIC VESICLES
MAPPING OF A DOMINANT IMMUNOGENIC REGION OF SYNAPTOPHYSIN, A MAJOR MEMBRANE-PROTEIN OF SYNAPTIC VESICLES
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DOI：
10.1016/0014-5793(90)80591-6
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发表时间：
1990-02-26
期刊：
影响因子：
3.5
通讯作者：
BETZ, H
中科院分区：
文献类型：
--
作者：
KNAUS, P;BETZ, H
突触素是突触囊泡的主要膜蛋白。从cDNA序列推断的其跨膜拓扑结构预测了4个跨膜区和含有特征性五肽重复结构的羧基末端胞质尾。单克隆抗体（mAb）SY38与突触素的胞质结构域结合。通过使用对应于截短形式的胞质尾区的融合蛋白，其表位定位于重复结构中心的柔性片段。其他四种mAb（c7.1、c7.2、c7.3、c7.4）共享相同的表位，因此其作为该膜蛋白的主要免疫原性区域出现。
Synaptophysin is a major integral membrane protein of synaptic vesicles. Its transmembrane topology deduced from the cDNA sequence predicts 4 transmembrane regions and a carboxy‐terminal cytoplasmic tail containing a characteristic pentapeptide repeat structure. The monoclonal antibody (mAb), SY38, binds to a cytoplasmic domain of synaptophysin. By using fusion proteins corresponding to truncated forms of the cytoplasmic tail, its epitope was located to a flexible segment in the center of the repeat structure. Four other mAbs (c7.1, c7.2, c7.3, c7.4) share the same epitope, which thus emerges as the major immunogenic region of this membrane protein.