A transporter of 1‐aminocyclopropane‐1‐carboxylic acid affects thallus growth and fertility in Marchantia polymorpha
A transporter of 1‐aminocyclopropane‐1‐carboxylic acid affects thallus growth and fertility in Marchantia polymorpha
复制标题
1-氨基环丙烷-1-羧酸转运蛋白对地钱菌体生长和育性的影响
DOI：
10.1111/nph.18510
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发表时间：
2022
期刊：
影响因子：
9.4
通讯作者：
Van de Poel, Bram
中科院分区：
文献类型：
--
作者：
Li, Dongdong;Dierschke, Tom;Roden, Stijn;Chen, Kunsong;Bowman, John L.;Chang, Caren;Van de Poel, Bram
在种子植物中，1-氨基环丙烷-1-羧酸(ACC)是植物激素乙烯的前体，但也具有不依赖于乙烯的信号转导作用。非种子植物产生ACC，但不能有效地将其转化为乙烯。在拟南芥中，ACC由氨基酸转运蛋白、赖氨酸组氨酸转运蛋白1(LHT1)和LHT2转运。在非种子植物中，LHT同系物尚未确定。在这里，我们分离到一个ACC不敏感突变体(MPain)，该突变体在地钱中存在吸收ACC的缺陷。Main在MpLHT1编码序列中含有移码突变(1个 碱基缺失)，并由野生型MpLHT1转基因表达补充。此外，在CRISPR/Cas9-Mplht1基因敲除突变体中重新产生了ACC不敏感。我们发现MpLHT1还可以转运1-羟基脯氨酸和1-组氨酸。我们根据突变表型研究了MpLHT1在营养生长和生殖中的生理功能。与野生型植物相比，Mpain和Mplht1植株体型较小，形成的萌发杯较少。Mplht1突变体的生育力也降低，而且颈卵器衰老较早。这些发现表明，MpLHT1是一种ACC和氨基酸转运体INM。多形核具有多种生理功能。我们认为MpLHT1有助于ACC和其他氨基酸INM的动态平衡。多态生长和繁殖。
In seed plants, 1‐aminocyclopropane‐1‐carboxylic acid (ACC) is the precursor of the plant hormone ethylene but also has ethylene‐independent signaling roles. Nonseed plants produce ACC but do not efficiently convert it to ethylene. InArabidopsis thaliana, ACC is transported by amino acid transporters, LYSINE HISTIDINE TRANSPORTER 1 (LHT1) and LHT2. In nonseed plants,LHThomologs have been uncharacterized.Here, we isolated an ACC‐insensitive mutant (Mpain) that is defective in ACC uptake in the liverwortMarchantia polymorpha. Mpaincontained a frameshift mutation (1 bp deletion) in the MpLHT1coding sequence, and was complemented by expression of a wild‐type MpLHT1transgene. Additionally, ACC insensitivity was re‐created in CRISPR/Cas9‐Mplht1knockout mutants. We found that MpLHT1 can also transportl‐hydroxyproline andl‐histidine.We examined the physiological functions of MpLHT1in vegetative growth and reproduction based on mutant phenotypes. Mpainand Mplht1plants were smaller and developed fewer gemmae cups compared to wild‐type plants. Mplht1mutants also had reduced fertility, and archegoniophores displayed early senescence.These findings reveal that MpLHT1 serves as an ACC and amino acid transporter inM. polymorphaand has diverse physiological functions. We propose that MpLHT1 contributes to homeostasis of ACC and other amino acids inM. polymorphagrowth and reproduction.