Use of Digital Droplet PCR to Detect Mycobacterium tuberculosis DNA in Whole Blood-Derived DNA Samples from Patients with Pulmonary and Extrapulmonary Tuberculosis.
Use of Digital Droplet PCR to Detect Mycobacterium tuberculosis DNA in Whole Blood-Derived DNA Samples from Patients with Pulmonary and Extrapulmonary Tuberculosis.
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使用数字微滴 PCR 检测肺结核和肺外结核患者全血 DNA 样本中的结核分枝杆菌 DNA
DOI：
10.3389/fcimb.2017.00369
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发表时间：
2017
影响因子：
5.7
通讯作者：
Peng Y
中科院分区：
文献类型：
--
作者：
Yang J;Han X;Liu A;Bai X;Xu C;Bao F;Feng S;Tao L;Ma M;Peng Y
结核病（TB）是一种慢性传染病，几个世纪以来一直威胁着公众健康。结核病的临床诊断过程既费时又费力。近20年来，基于实时荧光的定量PCR（实时PCR）因其敏感性和特异性而成为临床结核病诊断的更好选择。最近，数字液滴 PCR (ddPCR) 已被开发出来，它可能是微生物检测中传统实时 PCR 的理想替代方案。在本研究中，我们旨在评估 ddPCR 和实时 PCR 检测低水平循环结核分枝杆菌 (MTB) DNA 的能力。该研究涉及测试全血样本中的 MTB DNA 目标（称为 IS6110）。血液样本取自 28 名肺结核患者、28 名肺外结核患者和 28 名健康个体。结果表明，ddPCR可用于测量低水平的MTB DNA，并且有潜力用于根据临床样本诊断肺结核和肺外结核。
Tuberculosis (TB) is a chronic infectious disease that has been threatening public health for many centuries. The clinical diagnostic procedure for TB is time-consuming and laborious. In the last 20 years, real-time fluorescence-based quantitative PCR (real-time PCR) has become a better alternative for TB diagnosis in clinics due to its sensitivity and specificity. Recently, digital droplet PCR (ddPCR) has been developed, and it might be an ideal alternative to conventional real-time PCR for microorganism detection. In this study, we aimed to assess the capacity of ddPCR and real-time PCR for detecting low levels of circulating Mycobacterium tuberculosis (MTB) DNA. The study involved testing whole blood samples for an MTB DNA target (known as IS6110). Blood samples were obtained from 28 patients with pulmonary TB, 28 patients with extrapulmonary TB, and 28 healthy individuals. The results show that ddPCR could be used to measure low levels of MTB DNA, and it has the potential to be used to diagnose pulmonary and extrapulmonary TB based on clinical samples.