Lack of immune response to methyl methacrylate in lymphocyte cultures.
Lack of immune response to methyl methacrylate in lymphocyte cultures.
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淋巴细胞培养物中缺乏对甲基丙烯酸甲酯的免疫反应。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
Mats Grönblad
中科院分区：
文献类型：
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作者：
S. Santavirta;Y. Konttinen;Ville Bergroth;Mats Grönblad
为了分析甲基丙烯酸甲酯是否是免疫惰性的，将外周血单核细胞与细粉碎的甲基丙烯酸甲酯一起培养。植物血凝素（PHA）凝集素、结核菌素（PPD）抗原的纯化蛋白衍生物和单独的培养基用作阳性和阴性对照。研究了培养第0、1、3和5天的淋巴细胞动力学。使用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法分析主要组织相容性复合物位点II抗原（MHC位点II抗原; Ia）和白细胞介素-2受体（IL-2 R; Tac）表达，使用3 H-胸苷掺入和β-闪烁计数分析淋巴细胞DNA合成。在培养第1天和第3天，在光学显微镜下观察到淋巴细胞和单核细胞附着在甲基丙烯酸甲酯颗粒上。然而，结果显示没有甲基丙烯酸甲酯诱导的DNA合成，尽管记录了甲基丙烯酸甲酯诱导的MHC基因座II抗原和IL-2 R活化标志物表达;值得注意的是，这种表达不如PHA或PPD抗原驱动的淋巴细胞应答中观察到的明显。结果表明，甲基丙烯酸甲酯基本上是一种免疫惰性植入材料。然而，它似乎诱导炎性单核细胞迁移和粘附，导致轻微的非特异性淋巴细胞反应。
To analyze whether or not methyl methacrylate is immunologically inert, peripheral blood mononuclear cells were cultured with finely pulverized methyl methacrylate. Phytohemagglutinin (PHA) lectin, purified protein derivate of tuberculin (PPD) antigen, and culture medium alone were used as positive and negative controls. Lymphocyte kinetics on culture Days 0, 1, 3, and 5 were studied. Major histocompatibility complex locus II antigen (MHC locus II antigen; Ia) and interleukin-2 receptor (IL-2R; Tac) expression were analyzed using the avidin-biotin-peroxidase complex (ABC) method and lymphocyte DNA synthesis using 3H-thymidine incorporation and beta-scintillation counting. On culture Days 1 and 3, lymphocytes and monocytes were seen under the light microscope to be attached to methyl methacrylate particles. However, the results disclosed no methyl methacrylate-induced DNA synthesis, although methyl methacrylate-induced MHC locus II antigen and IL-2R activation marker expression were recorded; notably, this expression was less pronounced than that seen in PHA or PPD antigen driven lymphocyte response. The results suggest that methyl methacrylate is essentially an immunologically inert implant material. However, it seems to induce inflammatory mononuclear cell migration and adhesions leading to slightly nonspecific lymphocyte reaction.