Bio-functionalized silk hydrogel microfluidic systems.
Bio-functionalized silk hydrogel microfluidic systems.
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DOI：
10.1016/j.biomaterials.2016.03.041
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发表时间：
2016-07
期刊：
影响因子：
14
通讯作者：
Siwei Zhao;Ying Chen;Benjamin P. Partlow;Annie Golding;P. Tseng;J. Coburn;M. Applegate;J. Moreau;F. Omenetto;D. Kaplan
中科院分区：
文献类型：
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作者：
Siwei Zhao;Ying Chen;Benjamin P. Partlow;Annie Golding;P. Tseng;J. Coburn;M. Applegate;J. Moreau;F. Omenetto;D. Kaplan
基于丝蛋白水凝胶弹性体材料开发了生物功能化微流控系统。采用明胶牺牲成型和逐层组装的方法，在丝素水凝胶中构建了互连的三维微通道网络，最小特征分辨率为100 μm。实施机械激活阀以证明微流的气动控制。丝水凝胶微流体表现出可控的机械性能，在各种环境条件下的长期稳定性，除了光学透明度之外，还具有可调的体外生物降解性，为细胞/组织相关应用提供了比传统聚二甲基硅氧烷（PDMS）和现有的基于水凝胶的微流体选项独特的功能。正如在这里的工作中所证明的那样，在环境条件下的全水基制造过程使得在设备制造期间能够将活性生物物质掺入这些新的丝微流体系统的体相中，包括酶和活细胞，它们能够与微通道中的流体流动相互作用。这些基于丝水凝胶的微流控系统为工程化主动诊断设备、可在体内集成的组织和器官以及芯片上细胞传感系统提供了新的机会。
Bio-functionalized microfluidic systems were developed based on a silk protein hydrogel elastomeric materials. A facile multilayer fabrication method using gelatin sacrificial molding and layer-by-layer assembly was implemented to construct interconnected, three dimensional (3D) microchannel networks in silk hydrogels at 100 μm minimum feature resolution. Mechanically activated valves were implemented to demonstrate pneumatic control of microflow. The silk hydrogel microfluidics exhibit controllable mechanical properties, long-term stability in various environmental conditions, tunablein vitroandin vivodegradability in addition to optical transparency, providing unique features for cell/tissue-related applications than conventional polydimethylsiloxane (PDMS) and existing hydrogel-based microfluidic options. As demonstrated in the work here, the all aqueous-based fabrication process at ambient conditions enabled the incorporation of active biological substances in the bulk phase of these new silk microfluidic systems during device fabrication, including enzymes and living cells, which are able to interact with the fluid flow in the microchannels. These silk hydrogel-based microfluidic systems offer new opportunities in engineering active diagnostic devices, tissues and organs that could be integratedin vivo, and for on-chip cell sensing systems.