Smc5/6 complex regulates Sgs1 recombination functions.
Smc5/6 complex regulates Sgs1 recombination functions.
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DOI：
10.1007/s00294-016-0648-5
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发表时间：
2017-06
期刊：
影响因子：
2.5
通讯作者：
Aragon L
中科院分区：
文献类型：
--
作者：
Bermúdez-López M;Aragon L
RecQ解旋酶家族在从细菌到人类的进化中是保守的，并且在基因组稳定性中起关键作用。芽殖酵母RecQ解旋酶Sgs 1作为STR复合体（Sgs 1-Top3-Rmi 1）的一部分，在通过同源重组修复DNA损伤的过程中参与了几个关键过程。关于Sgs 1如何在受损部位招募和调节的信息有限。最近，我们和其他人发现了Smc 5/6复合物和Sgs 1之间的直接联系。Sgs 1在重组过程中的大多数作用，包括DNA末端切除、假日连接溶解和交叉抑制，都是通过Mms 21依赖的SUMO化来调节的。Smc 5/6首先作为STR的招募平台，然后SUMO化STR组分以调节其功能。重要的是，STR的组装完全独立于Smc 5/6。在这里，我们提供了一个简短的概述STR调控Smc 5/6。本文的在线版本（doi：10.1007/s 00294 -016-0648-5）包含补充材料，可供授权用户使用。
The family of RecQ helicases is evolutionary conserved from bacteria to humans and play key roles in genome stability. The budding yeast RecQ helicase Sgs1 has been implicated in several key processes during the repair of DNA damage by homologous recombination as part of the STR complex (Sgs1-Top3-Rmi1). Limited information on how is Sgs1 recruited and regulated at sites of damage is available. Recently, we and others have uncover a direct link between the Smc5/6 complex and Sgs1. Most roles of Sgs1 during recombination, including DNA end resection, Holiday junction dissolution, and crossover suppression, are regulated through Mms21-dependent SUMOylation. Smc5/6 first acts as a recruiting platform for STR and then SUMOylates STR components to regulate their function. Importantly, the assembly of STR is totally independent of Smc5/6. Here, we provide a brief overview of STR regulation by Smc5/6. The online version of this article (doi:10.1007/s00294-016-0648-5) contains supplementary material, which is available to authorized users.