Disruption of cytoplasmic microtubules by ultraviolet radiation.
Disruption of cytoplasmic microtubules by ultraviolet radiation.
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紫外线辐射破坏细胞质微管。
DOI：
10.1016/0014-4827(91)90527-2
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发表时间：
1991
影响因子：
3.7
通讯作者：
Chou,IN
中科院分区：
文献类型：
--
作者：
Zamansky,GB;Perrino,BA;Chou,IN
紫外线(UV)照射培养的人皮肤成纤维细胞会导致其微管解体。利用间接免疫荧光显微镜，我们现在已经研究了微管前体池的损伤是否可能导致微管的破坏。在细胞从冷处理中恢复过程中，暴露在多色紫外线辐射下会抑制微管的重新组装。此外，紫杉醇在紫外线照射的细胞中促进微管聚合和捆绑的能力受到抑制。然而，紫杉醇处理的细胞或洗涤剂提取的微管中的紫外线照射并不能破坏微管网络。这些数据表明，二聚体微管蛋白或聚合所需的另一种可溶性因子(S)的损伤，有助于紫外线照射下的人皮肤成纤维细胞微管的解体。
Ultraviolet (UV) irradiation of cultured human skin fibroblasts causes the disassembly of their microtubules. Using indirect immunofluorescence microscopy, we have now investigated whether damage to the microtubule precursor pool may contribute to the disruption of microtubules. Exposure to polychromatic UV radiation inhibits the reassembly of microtubules during cellular recovery from cold treatment. In addition, the ability of taxol to promote microtubule polymerization and bundling is inhibited in UV-irradiated cells. However, UV irradiation of taxol-pretreated cells orin situdetergent-extracted microtubules fails to disrupt the microtubule network. These data suggest that damage to dimeric tubulin, or another soluble factor(s) required for polymerization, contributes to the disassembly of microtubules in UV-irradiated human skin fibroblasts.