Recognition of a bicoid mRNA localization signal by a protein complex containing swallow, Nod, and RNA binding proteins
Recognition of a bicoid mRNA localization signal by a protein complex containing swallow, Nod, and RNA binding proteins
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DOI：
10.1016/s1534-5807(02)00397-0
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发表时间：
2003-01-01
影响因子：
11.8
通讯作者：
Macdonald, PM
中科院分区：
文献类型：
--
作者：
Arn, EA;Cha, BJ;Macdonald, PM
MRNAs的定位是果蝇胚胎体模式形成所必需的过程，需要负责运动和锚定的细胞组件识别顺式作用信号。我们已经纯化了一个大型的多蛋白复合体，它以一种特异的和对失活突变敏感的方式结合了最小形式的双核mRNA定位信号。已鉴定的复杂成分包括RNA结合蛋白Modulo、PABP和Smooth，已知的定位因子Swallow和Kinesin家族成员Nod。我们证明BCD基因在模变异体中的定位是有缺陷的。识别复合体中存在三个必需的定位成分(燕子、模数和特定的RNA结合活性)，这强烈地表明它与mRNA定位有关。
Localization of mRNAs, a process essential for embryonic body patterning in Drosophila, requires recognition of cis-acting signals by cellular components responsible for movement and anchoring. We have purified a large multiprotein complex that binds a minimal form of the bicoid mRNA localization signal in a manner both specific and sensitive to inactivating mutations. Identified complex components include the RNA binding proteins Modulo, PABP, and Smooth, the known localization factor Swallow, and the kinesin family member Nod. We demonstrate that localization of bcd mRNA is defective in modulo mutants. The presence of three required localization components (Swallow, Modulo, and specific RNA binding activity) within the recognition complex strongly implicates it in mRNA localization.