Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation
Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation
复制标题
基于磁分离的多功能核酸提取装置及其与液体处理系统配合进行高通量样品制备
DOI：
10.1166/jnn.2016.12583
复制
发表时间：
2016-07
影响因子：
--
通讯作者：
Nongyue He
中科院分区：
文献类型：
--
作者：
Zhu Chen;Libo Nie;Bin Liu;Nongyue He
磁性纳米粒子（MNP）在样品制备中的应用受到了广泛的关注。MNP的易操作性为实验室自动化提供了一个很有前途的解决方案。基于磁分离技术的实验室机器人和工作站正处于快速发展阶段，其在临床上的实际应用受到越来越多的关注。为了提高处理通量并降低交叉污染的风险，我们开发了一种多功能模块来优化自动化工作站中磁性核酸分离的工作流程。该装置将磁分离、加热和振荡三大功能巧妙地结合在一起，无需将96孔板转移到其他辅助模块，即可在现场完成样品制备。在该平台上进行了测试实验。实验结果表明，该多功能移液装置能够很好地与移液系统配合使用，能够满足高通量样品制备的要求。
The use of magnetic nanoparticles (MNPs) in sample preparation has gained much attention. Easy-to-handle property of MNPs has provided a great promising solution for lab automation. Laboratory robotics and workstations based on magnetic separation are in rapid development process, and increasing efforts are debited for their practical applications in clinical methods. To increase the processing throughput and reduce the risk of cross contamination, we developed a multifunctional module to optimize the workflow of magnetic nucleic acid separation in automated workstation. Three functions (magnetic separation, heating, and shaking) were elaborately combined into this device, by which the 96-well plate-based sample preparation can be completed on site without transferring the plate to other auxiliary modules. Test experiments were carried out on this platform. It was shown that this multifunctional device could cooperate well with our pipetting system, and this new platform exhibited its ability to meet the demands for high-throughput sample preparation.