Affibody modified and radiolabeled gold-iron oxide hetero-nanostructures for tumor PET, optical and MR imaging.
Affibody modified and radiolabeled gold-iron oxide hetero-nanostructures for tumor PET, optical and MR imaging.
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DOI：
10.1016/j.biomaterials.2013.01.014
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发表时间：
2013-04
期刊：
影响因子：
14
通讯作者：
Cheng, Zhen
中科院分区：
文献类型：
--
作者：
Yang, Meng;Cheng, Kai;Qi, Shibo;Liu, Hongguang;Jiang, Yuxin;Jiang, Han;Li, Jinbo;Chen, Kai;Zhang, Huimao;Cheng, Zhen
在一个结构内具有两种不同功能纳米材料（金（Au）和氧化铁（Fe 3 O 4，IO））的高度单分散的异质纳米结构被成功开发为用于表皮生长因子受体（EGFR）阳性肿瘤成像的基于亲和体的三峰纳米探针（正电子发射断层扫描，PET;光学成像;和磁共振成像，MRI）。与其他单一组分的常规纳米结构不同，Au-IO异质纳米结构（Au-IONP）具有独特的化学和物理性质，能够将联合收割机几种成像方式结合在一起以提供互补信息。异质纳米结构内的IO组分用作MRI的T2报告物;并且金组分用作光学和PET报告物。此外，这样的异源纳米探针可以以高效和可靠的方式为靶向分子（抗EGFR亲和体蛋白）和PET成像报告分子（放射性金属64 Cu螯合剂）两者的表面特异性修饰提供稳健的纳米平台。体外和体内研究表明，所得的纳米探针在人EGFR表达细胞和肿瘤中为PET和MRI成像提供了高特异性、灵敏度和优异的肿瘤对比度。我们的研究数据还强调了异质纳米颗粒的EGFR靶向效率及其进一步治疗诊断应用的可行性。
A highly monodispersed hetero-nanostructure with two different functional nanomaterials (gold (Au) and iron oxide (Fe3O4, IO)) within one structure was successfully developed as Affibody based trimodality nanoprobe (positron emission tomography, PET; optical imaging; and magnetic resonance imaging, MRI) for imaging of epidermal growth factor receptor (EGFR) positive tumors. Unlike other regular nanostructures with a single component, the Au-IO hetero-nanostructures (Au-IONPs) with unique chemical and physical properties have capability to combine several imaging modalities together to provide complementary information. The IO component within hetero-nanostructures serve as a T2 reporter for MRI; and gold component serve as both optical and PET reporters. Moreover, such hetero-nanoprobes could provide a robust nano-platform for surface-specific modification with both targeting molecules (anti-EGFR Affibody protein) and PET imaging reporters (radiometal 64Cu chelators) in highly efficient and reliable manner. In vitro and in vivo study showed that the resultant nanoprobe provided high specificity, sensitivity, and excellent tumor contrast for both PET and MRI imaging in the human EGFR-expressing cells and tumors. Our study data also highlighted the EGFR targeting efficiency of hetero-nanoparticles and the feasibility for their further theranostic applications.
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影响因子：
15
作者：
Liu, Wenhao;Howarth, Mark;Bawendi, Moungi G.
通讯作者：
Bawendi, Moungi G.
DOI：
10.2967/jnumed.111.100842
发表时间：
2012-07
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
Miao Z;Ren G;Liu H;Qi S;Wu S;Cheng Z
通讯作者：
Cheng Z
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
9.3
作者：
Delso, Gaspar;Fuerst, Sebastian;Ziegler, Sibylle I.
通讯作者：
Ziegler, Sibylle I.
影响因子：
3.5
作者：
Miao, Zheng;Ren, Gang;Cheng, Zhen
通讯作者：
Cheng, Zhen