Intracellular dynamics and fate of polystyrene nanoparticles in A549 Lung epithelial cells monitored by image (cross-) correlation spectroscopy and single particle tracking
Intracellular dynamics and fate of polystyrene nanoparticles in A549 Lung epithelial cells monitored by image (cross-) correlation spectroscopy and single particle tracking
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DOI：
10.1016/j.bbamcr.2015.07.004
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发表时间：
2015-10-01
影响因子：
5.1
通讯作者：
Ameloot, Marcel
中科院分区：
文献类型：
--
作者：
Deville, Sarah;Penjweini, Rozhin;Ameloot, Marcel
通过研究纳米粒子的时空行为，可以获得对纳米粒子在细胞中的摄取途径、胞内运输和亚细胞靶向的新认识。在这项工作中，我们提出了应用图像（交叉）相关光谱（IC（C）S）和单粒子跟踪（SPT）监测聚苯乙烯（PS）纳米粒子在人肺癌A549细胞系的细胞内动力学。利用时间和时空图像相关光谱（TICS和STICS）对细胞内纳米粒子的整体动力学行为进行了表征。此外，通过监测单个NP，通过SIT获得了对NP运动中检测到的扩散和流动的更直接的解释。这两种技术都表明PS NP在A549细胞中的转运主要依赖于微管辅助转运。应用时空图像互相关光谱技术（STICCS），识别了核蛋白与早期核内体、晚期核内体和溶酶体的相关运动。在实验的时间间隔期间，PS NP在内溶酶体隔室中均匀分布。与其他细胞器相比，NPs与溶酶体的共转运显著更大。在本研究中，我们表明，基于ICS的技术和SPT的互补性，使一个一致的复杂行为的生物系统内的NP的精细模型。(C)2015爱思唯尔B.V.保留所有权利。
Novel insights in nanoparticle (NP) uptake routes of cells, their intracellular trafficking and subcellular targeting can be obtained through the investigation of their temporal and spatial behavior. In this work, we present the application of image (cross-) correlation spectroscopy (IC(C)S) and single particle tracking (SPT) to monitor the intracellular dynamics of polystyrene (PS) NPs in the human lung carcinoma A549 cell line. The ensemble kinetic behavior of NPs inside the cell was characterized by temporal and spatiotemporal image correlation spectroscopy (TICS and STICS). Moreover, a more direct interpretation of the diffusion and flow detected in the NP motion was obtained by SIT by monitoring individual NPs. Both techniques demonstrate that the PS NP transport in A549 cells is mainly dependent on microtubule-assisted transport. By applying spatiotemporal image cross-correlation spectroscopy (STICCS), the correlated motions of NPs with the early endosomes, late endosomes and lysosomes are identified. PS NPs were equally distributed among the endolysosomal compartment during the time interval of the experiments. The cotransport of the NPs with the lysosomes is significantly larger compared to the other cell organelles. In the present study we show that the complementarity of ICS-based techniques and SPT enables a consistent elaborate model of the complex behavior of NPs inside biological systems. (C) 2015 Elsevier B.V. All rights reserved.