Characterization of the sol operon in butanol-hyperproducing Clostridium saccharoperbutylacetonicum strain N1-4 and its degeneration mechanism
Characterization of the sol operon in butanol-hyperproducing Clostridium saccharoperbutylacetonicum strain N1-4 and its degeneration mechanism
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DOI：
10.1271/bbb.60370
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发表时间：
2007-01-01
影响因子：
1.6
通讯作者：
Furukawa, Kensuke
中科院分区：
文献类型：
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作者：
Kosaka, Tomoyuki;Nakayama, Shunichi;Furukawa, Kensuke
从糖过丁基乳酸梭菌N1-4中克隆出由bld、ctfA、ctfB和adc组成的溶剂型溶胶操纵子。这些基因与beijerinckii Clostridium NCIMB 8052对应的sol基因相似度高达95-98%。n1 - 4sol基因簇在两个启动子的控制下以多顺反子方式转录，其转录在溶剂生成过程中受到高度诱导。由N1-4衍生的退化菌株DGN34保持了sol基因，但DGN3-4的sol操纵子在溶剂形成过程中几乎没有被诱导转录。从野生型N1-4的培养上清中提取的物质使我们能够诱导DGN3-4中sol操纵子的转录。这些结果表明，这种退化是由于sol操纵子的诱导机制失效造成的，转录可能受到群体感应机制的控制。
The solventogenic sol operon consisting of bld, ctfA, ctfB, and adc was cloned from Clostridium saccharoperbutylacetonicum strain N1-4. These genes share as high as 95-98% similarity with the corresponding sol genes of Clostridium beijerinckii NCIMB 8052. The N1-4 sol gene cluster was transcribed in a polycistronic manner under the control of two promoters, and its transcription was highly induced during solventogenesis. Strain DGN34, the degenerated strain derived from N1-4, maintained the sol genes, but transcription of the DGN3-4 sol operon was hardly induced during solventogenesis. A substance extracted from the culture supernatants of wild-type N1-4 allowed us to induce transcription of the sol operon in DGN3-4. These results suggest that the degeneration is caused by the incompetence of the induction mechanism of the sol operon, and that transcription might be under the control of a quorum-sensing mechanism.