Variations in the Coding Region of the Agouti Signaling Protein Gene Do Not Explain Agouti/Non-agouti Phenotypes in Macaques
Variations in the Coding Region of the Agouti Signaling Protein Gene Do Not Explain Agouti/Non-agouti Phenotypes in Macaques
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DOI：
10.1007/s10914-010-9137-7
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发表时间：
2010-04
影响因子：
1.9
通讯作者：
Kazuhiro Nakayama;T. Shotake;Osamu Takeneka;T. Ishida
中科院分区：
文献类型：
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作者：
Kazuhiro Nakayama;T. Shotake;Osamu Takeneka;T. Ishida
Agglutination是哺乳动物包括灵长类动物中常见的色素沉着表型。已知聚集蛋白信号蛋白基因（ASIP）的突变会导致实验室小鼠的非聚集蛋白黑发。目前还不清楚ASIP基因的序列变异是否与猕猴（GenusMacaca）的agglutinase/non-agglutinase表型有关。为了解决这一问题，我们测定并比较了18种猕猴ASIPin蛋白编码区的核苷酸序列，鉴定出16种不同的ASIP序列，显示黄色无毛的猕猴nemestrina与几种非无毛的猕猴具有相同的ASIP氨基酸序列。在非聚集性深色毛色的猕猴中，未发现ASI功能重要位点的序列变化。这些结果表明，ASIP蛋白编码区的变异不能解释猕猴的非聚集性深色毛色。ASIP和其他参与色素系统的基因的上游调控区在猕猴毛色变异中的作用有待进一步研究。
Agouti is a common pigmentation phenotype in mammals including primates. Mutations in the agouti signaling protein gene (ASIP) are known to result in non-agouti black hairs in laboratory mice. It is still unclear whether sequence variation inASIPis linked with the agouti/non-agouti phenotypes in macaques (GenusMacaca). To address this issue, we have determined and compared nucleotide sequences of protein coding region ofASIPin 18 macaque species and have identified 16 different sequences of theASIP.Macaca nemestrina,which showed yellow agouti hairs, shared an identical amino acid sequence ofASIPwith several non-agouti species. No sequence changes were found in functionally important sites ofASIPin the macaques showing non-agouti dark hair color. These results indicated that the variation in the protein coding region ofASIPdid not explain the non-agouti dark coat color in the macaques. Upstream regulatory regions ofASIPand other genes participating in pigmentation system remain to be investigated for the hair color variation in the macaques.