What Are 3′ UTRs Doing?
What Are 3′ UTRs Doing?
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DOI：
10.1101/cshperspect.a034728
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发表时间：
2019-10-01
影响因子：
7.2
通讯作者：
Mayr, Christine
中科院分区：
文献类型：
--
作者：
Mayr, Christine
信使RNA(Messenger RNAs，mRNAs)的3‘非翻译区(3’UTRs)是已知的调节基于信使RNA的过程，如信使RNA的定位、信使RNA的稳定性和翻译。此外，3‘非编码区还可以建立3’非编码区介导的蛋白质-蛋白质相互作用(PPI)，从而将3‘非编码区编码的遗传信息传递给蛋白质。这一功能已被证明可以调节不同的蛋白质功能，包括蛋白质复合体的形成或翻译后修饰，但也有望改变蛋白质的构象。因此，3‘端非编码区介导的信息传递可以调节氨基酸序列中没有编码的蛋白质特征。这篇综述总结了3‘非编码区的两种功能--基于mRNA和蛋白质的过程的调节--并强调了每种3’非编码区功能是如何被发现的，重点是所使用的实验方法和所学的概念。本综述还讨论了利用最近的技术进步来研究3‘非编码区功能的新方法。
3' untranslated regions (3' UTRs) of messenger RNAs (mRNAs) are best known to regulate mRNA-based processes, such as mRNA localization, mRNA stability, and translation. In addition, 3' UTRs can establish 3' UTR-mediated protein-protein interactions (PPIs), and thus can transmit genetic information encoded in 3' UTRs to proteins. This function has been shown to regulate diverse protein features, including protein complex formation or posttranslational modifications, but is also expected to alter protein conformations. Therefore, 3' UTR-mediated information transfer can regulate protein features that are not encoded in the amino acid sequence. This review summarizes both 3' UTR functions-the regulation of mRNA and protein-based processes-and highlights how each 3' UTR function was discovered with a focus on experimental approaches used and the concepts that were learned. This review also discusses novel approaches to study 3' UTR functions in the future by taking advantage of recent advances in technology.