CONSENSUS SEQUENCE FOR ESCHERICHIA-COLI HEAT-SHOCK GENE PROMOTERS
CONSENSUS SEQUENCE FOR ESCHERICHIA-COLI HEAT-SHOCK GENE PROMOTERS
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DOI：
10.1073/pnas.82.9.2679
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
GROSS, CA
中科院分区：
文献类型：
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作者：
COWING, DW;BARDWELL, JCA;GROSS, CA
鉴定了大肠杆菌热休克操纵子dnaK和groE的启动子以及编码热休克蛋白C62.5的基因。每个启动子的转录在体内都是热诱导的，并且每个启动子都被含有。sigma的RNA聚合酶在体外识别。32, sigma。由rpoH （htpR）编码而不是由含有。sigma.70的RNA聚合酶编码的因子。通过对热休克启动子序列的比较，提出了一个一致的启动子序列，即t- n -t- c- n -c- c- c- c- t- t- g -a位于-35区，c- c- c- a -t- t- t- a位于-10区。这些序列不同于含有.sigma的全酶所识别的一致序列。70，少校。西格玛。在大肠杆菌中这表明，由不同形式的全酶识别的启动子序列的累积共识序列与一个模型是相容的。只识别启动子的-10区域。
Promoters for the E. coli heat shock operons dnaK and groE and the gene encoding heat shock protein C62.5 were identified. Transcription from each promoter is heat-inducible in vivo, and each is recognized in vitro by RNA polymerase containing .sigma.32, the .sigma. factor encoded by rpoH (htpR) but not by RNA polymerase containing .sigma.70. The sequences of the heat shock promoters were compared and a consensus promoter sequence, having T-N-t-C-N-C-c-C-T-T-G-A-A in the -35 region and C-C-C-C-A-T-t-T-a in the -10 region was proposed. These sequences differ from the consensus sequence recognized by holoenzyme containing .sigma.70, the major .sigma. in E. coli. It is suggested that the accumulated consensus sequences of promoters recognized by alternate forms of holoenzyme are compatible with a model in which .sigma. recognizes only the -10 region of the promoter.