Titania-coated monolithic silica as separation medium for high performance liquid chromatography of phosphorus-containing compounds
Titania-coated monolithic silica as separation medium for high performance liquid chromatography of phosphorus-containing compounds
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DOI：
10.1002/jssc.200401932
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发表时间：
2005-01-01
影响因子：
3.1
通讯作者：
Nakanishi, K
中科院分区：
文献类型：
--
作者：
Miyazaki, S;Miah, MY;Nakanishi, K
报道了一种制备二氧化钛涂层硅胶整体固定相的方法，用于含磷化合物的液相色谱分离。二氧化钛涂层的二氧化硅柱表现出有效的分离与低压降，这是一个典型的整体结构的特征，也具有磷的选择性，这是一个独特的性质的二氧化钛表面。考察了二氧化钛包覆硅胶整体柱的材料特性，并将树脂包覆硅胶整体柱用于磷酸化化合物的高效液相色谱分析。磷酸化物质的高效分离表明，新型二氧化钛涂层整体硅胶柱将发现作为一个有用的工具，在生物化学领域的应用，特别是在后基因组分析。
A method of preparing titania-coated monolithic silica stationary phase has been developed to achieve liquid chromatographic separation of phosphorus-containing compounds, which have recently been attracting increasing attention in biochemical research. The titania-coated silica columns exhibited efficient separation with low pressure drop, which is a typical feature of monolithic structures, and also possessed phospho-selectivity, which is a unique property of the titania surface. The material characteristics of titania-coated monolithic silica were examined, and then resin-clad columns were applied to the HPLC analysis of phosphorylated compounds. Highly efficient separation of phosphorylated substances indicated that the novel titania-coated monolithic silica column will find applications as a useful tool in the field of biochemistry, especially in post-genomic analyses.