Expressions of sonic hedgehog, patched, smoothened and Gli-1 in human intestinal stromal tumors and their correlation with prognosis.
Expressions of sonic hedgehog, patched, smoothened and Gli-1 in human intestinal stromal tumors and their correlation with prognosis.
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DOI：
10.3748/wjg.v12.i35.5687
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发表时间：
2006-09
影响因子：
4.3
通讯作者：
A. Yoshizaki;T. Nakayama;S. Naito;C. Wen;I. Sekine
中科院分区：
文献类型：
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作者：
A. Yoshizaki;T. Nakayama;S. Naito;C. Wen;I. Sekine
目的探讨包括sonic hedgehog （Shh）、Patched （Ptc）、Smoothened （Smo）和gli1在内的hedgehog （Hh）信号通路在人胃肠道间质瘤（gist）中的作用。方法采用免疫组化染色法检测gist、平滑肌瘤和神经鞘瘤患者手术切除标本中hedgehog信号成分Shh、Ptc、Smo和gli1的异常表达。结果31例gist标本中Shh、Ptc、Smo和gli1的阳性分别为58.1%（18 / 31）、77.4%（24 / 31）、80.6%（25 / 31）和58.1%（18 / 31）。在平滑肌瘤中，Shh、Ptc、Smo和gli1分别为92.3%（12 / 13）、92.3%（12 / 13）、69.2%（9 / 13）和92.3%（12 / 13）呈阳性。在神经鞘瘤中，Shh、Ptc、Smo和gli1的阳性分别为83.3%（5 / 6）、83.3%（5 / 6）、83.3%（5 / 6）和100%（6 / 6）。免疫组化结果显示，Shh和gli1在平滑肌瘤组织中的表达明显高于gist组织（P < 0.05）。Shh的表达与肿瘤危险类别的分级及肿瘤大小呈极显著相关（P < 0.05）。然而，Ptc和Smo的表达与组织病理分化无关。结论Hh信号通路可能在人肠间质瘤的成肌分化和恶性潜能中发挥重要作用。
AIM To investigate the role that the hedgehog (Hh) signaling pathway, which includes sonic hedgehog (Shh), Patched (Ptc), Smoothened (Smo) and Gli-1, plays in human gastrointestinal stromal tumors (GISTs). METHODS Surgically resected specimens from patients with GISTs, leiomyomas and schwannomas were examined by immunohistochemical staining for aberrant expression of hedgehog signaling components, Shh, Ptc, Smo and Gli-1, respectively. RESULTS In GISTs, 58.1% (18 of 31), 77.4% (24 of 31), 80.6% (25 of 31) and 58.1% (18 of 31) of the specimens stained positive for Shh, Ptc, Smo and Gli-1, respectively. In leiomyomas, 92.3% (12 of 13), 92.3% (12 of 13), 69.2% (9 of 13) and 92.3% (12 of 13) stained positive for Shh, Ptc, Smo and Gli-1, respectively. In schwannomas, 83.3% (5 of 6), 83.3% (5 of 6), 83.3% (5 of 6) and 100% (6 of 6) stained positive for Shh, Ptc, Smo and Gli-1, respectively. Immunohistochemistry revealed that the expressions of Shh and Gli-1 were significantly higher in leiomyomas than in GISTs (P < 0.05, respectively). Shh expression strongly correlated with the grade of tumor risk category and with tumor size (P < 0.05, respectively). However, the expressions of Ptc and Smo did not correlate with histopathological differentiation. CONCLUSION These results suggest that the Hh signaling pathway may play an important role in myogenic differentiation and the malignant potential of human intestinal stromal tumors.