A consensus RNA signal that directs germ layer determinants to the vegetal cortex of Xenopus oocytes
A consensus RNA signal that directs germ layer determinants to the vegetal cortex of Xenopus oocytes
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DOI：
10.1006/dbio.2002.0719
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发表时间：
2002-08-01
影响因子：
2.7
通讯作者：
King, ML
中科院分区：
文献类型：
--
作者：
Bubunenko, M;Kress, TL;King, ML
RNA定位是早期胚胎中产生细胞多样性和极性的重要机制。在非洲爪蟾中，编码T盒转录因子VegT的RNA的正确定位对于内胚层和中胚层的正确空间组织和身份至关重要。尽管已经鉴定了许多定位RNA的3'UTR中的定位信号，但是对什么构成RNA定位信号的了解仍然是难以捉摸的。为了研究将不同RNA引导至相同亚细胞区域的信号之间可能的共同特征，我们对未表征的VegT RNA定位信号进行了详细分析，并将其与研究充分的Vg1定位信号进行了比较。这两种RNA在卵子发生的同一时期定位于植物皮层。我们的研究结果表明，一个共同的RNA定位信号在集群冗余蛋白结合基序和反式作用因子的水平。我们提出，RNA定位信号的特征一般不是核苷酸序列或二级结构本身，而是特定冗余蛋白质结合基序的关键聚类。(C)2002 Elsevier Science（美国）。
RNA localization is an important mechanism for generating cellular diversity and polarity in the early embryo. In Xenopus, the correct localization of the RNA encoding the T-box transcription factor VegT is essential for the correct spatial organization and identity of endoderm and mesoderm. Although localization signals in the 3' UTR have been identified for many localized RNAs, insight into what constitutes an RNA localization signal remains elusive. To investigate possible common features between signals that direct different RNAs to the same subcellular region, we carried out a detailed analysis of the uncharacterized VegT RNA localization signal and compared it with the well-studied Vg1 localization signal. Both RNAs localize to the vegetal cortex during the same period of oogenesis. Our results suggest a common RNA localization signal at the level of clustered redundant protein-binding motifs and trans-acting factors. We propose that what characterizes RNA localization signals in general is not the nucleotide sequence or secondary structure per se, but the critical clustering of specific redundant protein-binding motifs. (C) 2002 Elsevier Science (USA).