n-Butyrate inhibition of hyaluronate synthesis in cultured human fibroblasts.
n-Butyrate inhibition of hyaluronate synthesis in cultured human fibroblasts.
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正丁酸盐抑制培养的人成纤维细胞中透明质酸的合成。
DOI：
10.1172/jci112979
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
T. Smith
中科院分区：
文献类型：
--
作者：
T. Smith
在培养的人皮肤成纤维细胞中研究了短链脂肪族羧酸正丁酸对糖胺聚糖 (GAG) 积累的影响。正常成纤维细胞培养物生长至汇合，转移至不含或含有正丁酸盐的培养基中24小时，用[3H]乙酸盐或[3H]葡萄糖胺标记，并分析[3H]GAG和[3H]透明质酸盐的积累。低浓度 (0.1-1 mM) 会刺激积累高达 27%。较高浓度的正丁酸盐（大于 1 mM）可抑制 [3H]GAG 高达 70-90%。该效应在 10 mM 时达到最大，在 3 mM 时达到一半。丙酸盐具有类似的作用，但效力较弱。在结肠成纤维细胞中进行的平行研究表明，正丁酸盐也可以显着抑制该细胞类型中的 [3H]GAG 积累。这些作用是快速的，在治疗后 3 小时内发生，并且是可逆的。硫酸软骨素的积累不受该化合物的影响。脉冲追踪研究未能证明对 [3H]GAG 降解有任何影响。
The effects of the short-chain aliphatic carboxylic acid, n-butyrate, on glycosaminoglycan (GAG) accumulation were studied in cultured human skin fibroblasts. Normal fibroblast cultures were grown to confluence, shifted to a medium without or with n-butyrate for 24 h, labeled with either [3H]acetate or [3H]glucosamine and analyzed for [3H]GAG and [3H]hyaluronate accumulation. Accumulation was stimulated at low concentrations (0.1-1 mM) by up to 27%. Higher concentrations of n-butyrate (greater than 1 mM) inhibited [3H]GAG by up to 70-90%. This effect was maximal at 10 mM and half-maximal at 3 mM. Propionate had similar effects but was less potent. Parallel studies conducted in colonic fibroblasts revealed that n-butyrate could markedly inhibit [3H]GAG accumulation in that cell type as well. These effects were rapid, occurring within 3 h of treatment, and were reversible. Chondroitin sulfate accumulation was unaffected by the compound. A pulse-chase study failed to demonstrate any effect on [3H]GAG degradation.
甲状腺激素对人皮肤成纤维细胞糖胺聚糖积累的影响。
影响因子：
4.8
作者：
Smith,TJ;Horwitz,AL;Refetoff,S
通讯作者：
Refetoff,S
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Boffa,LC;Gruss,RJ;Allfrey,VG
通讯作者：
Allfrey,VG
少突胶质细胞瘤细胞中透明质酸合成酶的亚细胞定位。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Philipson,LH;Schwartz,NB
通讯作者：
Schwartz,NB