Cloning of canine gastrin cDNA's encoding variant amino acid sequences.
Cloning of canine gastrin cDNA's encoding variant amino acid sequences.
复制标题
犬胃泌素 cDNA 编码变异氨基酸序列的克隆。
作者：
Gantz,I;Takeuchi,T;Yamada,T
我们从犬胃窦粘膜cDNA文库中分离到两个编码胃泌素的克隆。克隆在编码区的2个位点的核苷酸序列不同。其中一个克隆证实了通过肽纯化和分析获得的狗胃泌素的氨基酸序列。在另一个克隆中的AG取代导致编码胃泌素的生物活性羧基末端部分的区域中的Thr-Ala取代。在cDNA序列中观察到的异质性的来源尚不清楚，但其位置可能与犬胃泌素的生理学有关。
We have isolated 2 clones encoding gastrin from a canine antral mucosa cDNA library. The clones differed in nucleotide sequence at 2 sites in the coding region. One of the clones confirmed the amino acid sequence of dog gastrin obtained by peptide purification and analysis. An AG substitution in the other clone resulted in a Thr-Ala substitution in the region encoding the biologically active carboxyl-terminal portion of gastrin. The source of the observed heterogeneity in cDNA sequences is not known but its location may have implications with regard to the physiology of gastrin in dogs.