Dissection of overlapping functions within the adenovirus type 5 E1A gene.
Dissection of overlapping functions within the adenovirus type 5 E1A gene.
复制标题
5 型腺病毒 E1A 基因内重叠功能的剖析。
DOI：
10.1002/j.1460-2075.1984.tb02066.x
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Shenk,T
中科院分区：
文献类型：
--
作者：
Winberg,G;Shenk,T
腺病毒E1A基因编码多个重叠的mRNA，其产物在病毒裂解周期中调节mRNA水平，并促进非允许细胞的转化。为了赋予E1A基因产物特定的功能，构建了两个5型腺病毒变体。突变体dl347和348分别携带与E1A 12和13S mrna对应的克隆片段，取代了正常的E1A基因。这些变异产生了预测的E1A特异性mrna和多肽。两种病毒都能在HeLa细胞中高效生长。虽然13S mRNA产物更有效，但两种mRNA的产物都可以刺激其他转录单位的mRNA积累。这两种病毒都能在已建立的大鼠细胞系中诱导转化灶的形成。两种病毒均不能在正常频率下转化原代大鼠胚胎细胞，诱导的dl347灶转化不完全或失败。因此，12S和13S mrna编码的功能对于大鼠原代细胞的高效和完整转化是必需的。
The adenovirus E1A gene encodes multiple, overlapping mRNAs whose products function both to regulate mRNA levels during the lytic cycle of the virus and to facilitate transformation of non‐permissive cells. To assign specific functions to the E1A gene products, two adenovirus type 5 variants have been constructed. Mutants dl347 and 348 carry cloned segments corresponding to the E1A 12 and 13S mRNAs, respectively, in place of the normal E1A gene. The variants produced the predicted E1A‐specific mRNAs and polypeptides. Both viruses grew efficiently in HeLa cells. Although the 13S mRNA products were more effective, the products of either mRNA species could stimulate the accumulation of mRNAs from additional transcription units. Both viruses could induce the formation of transformed foci in an established rat cell line. Neither virus could transform primary rat embryo cells at normal frequency, and the dl347 foci which were induced were incomplete or abortive transformants. Thus, functions encoded by both 12S and 13S mRNAs are required for efficient and complete transformation of primary rat cells.