CNT/Sericin Conductive Nerve Guidance Conduit Promotes Functional Recovery of Transected Peripheral Nerve Injury in a Rat Model
CNT/Sericin Conductive Nerve Guidance Conduit Promotes Functional Recovery of Transected Peripheral Nerve Injury in a Rat Model
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CNT/丝胶传导神经引导导管促进大鼠模型中横断周围神经损伤的功能恢复
DOI：
10.1021/acsami.0c08457
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发表时间：
2020-08-19
影响因子：
9.5
通讯作者：
Wang, Lin
中科院分区：
文献类型：
--
作者：
Li, Xiaolin;Yang, Wen;Wang, Lin
周围神经损伤通常会导致不良后果，如疼痛性神经病变和残疾。自体神经移植是目前的金标准，然而，有限的供体神经来源仍然是一个问题。许多组织工程神经引导导管已被开发作为自体移植物的替代品。然而，少数管道可以达到与自体移植物相当的修复效果。在这里，我们报告的开发和应用的碳纳米管（CNT）/丝胶神经导管的导电性和适当的机械性能的神经修复。这种碳纳米管/丝胶蛋白导管具有良好的性能，包括生物相容性，生物降解性，多孔微结构，和适当的溶胀性能。因此，我们在大鼠损伤模型中应用该导管桥接与电刺激（ES）相结合的横断坐骨神经的10 mm间隙缺损。12周后，我们观察到CNT/丝胶导管结合电刺激可以有效地促进结构修复和功能恢复，与自体移植物相当，通过形态学和组织学分析，电生理反应，功能研究和靶肌肉神经再支配评估证明。这些研究结果表明，这种导电CNT/丝胶导管结合电刺激可能有潜力作为一种新的替代修复横断周围神经。
Peripheral nerve injury usually leads to poor outcomes such as painful neuropathies and disabilities. Autogenous nerve grafting is the current gold standard; however, the limited source of a donor nerve remains a problem. Numerous tissue engineering nerve guidance conduits have been developed as substitutes for autografts. However, a few conduits can achieve the reparative effect equivalent to autografts. Here, we report for the development and application of a carbon nanotube (CNT)/sericin nerve conduit with electrical conductivity and suitable mechanical properties for nerve repair. This CNT/sericin conduit possesses favorable properties including biocompatibility, biodegradability, porous microarchitecture, and suitable swelling property. We thus applied this conduit for bridging a 10 mm gap defect of a transected sciatic nerve combined with electrical stimulation (ES) in a rat injury model. By the end of 12 weeks, we observed that the CNT/sericin conduit combined with electrical stimulation could effectively promote both structural repair and functional recovery comparable to those of the autografts, evidenced by the morphological and histological analyses, electrophysiological responses, functional studies, and target muscle reinnervation evaluations. These findings suggest that this electric conductive CNT/sericin conduit combined with electrical stimulation may have the potential to serve as a new alternative for the repair of transected peripheral nerves.