Biological in vitro effects of fibrin glue: Fibroblast proliferation, expression and binding of growth factors
Biological in vitro effects of fibrin glue: Fibroblast proliferation, expression and binding of growth factors
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DOI：
10.1080/00365520410003371
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发表时间：
2004-10-01
影响因子：
1.9
通讯作者：
Pohle, T
中科院分区：
文献类型：
--
作者：
Becker, JC;Domschke, W;Pohle, T
背景：纤维蛋白胶用于内镜下出血性溃疡的治疗。与其他注射技术相比，这种治疗有加速溃疡闭合的记录；然而，生物学上的原因尚不清楚。方法：通过体外模型比较纤维蛋白胶对胃上皮细胞（AGS, KATO III）和间充质细胞（成纤维细胞）生长因子的表达和分泌的影响，以及它们与其他基质的增殖反应和相互作用。结果：天然纤维蛋白胶不释放血管内皮生长因子（VEGF），但能够在152.6 pg/mL的生物学相关浓度下结合该生长因子。在I型胶原基质中加入纤维蛋白胶可增加胃壁成纤维细胞的增殖率。上皮细胞中VEGF和血小板衍生生长因子（PDGF） mRNA转录显著升高。在含有基质和上皮细胞的纤维蛋白胶上培养成纤维细胞，两种细胞系的VEGF分泌均增加。结论：纤维蛋白胶导致成纤维细胞增殖增加，VEGF局部积聚。这些发现至少可以部分解释纤维蛋白胶注射治疗出血性溃疡的加速愈合。
Background: Fibrin glue is used in the endoscopic therapy of bleeding ulcerations. Accelerated closure of ulcers has been documented for this treatment in comparison with other injection techniques; the biological reason, however, remains unclear. Methods: In an in vitro model the effects of fibrin glue on the expression and secretion of growth factors by gastric epithelial (AGS, KATO III) and mesenchymal cells ( fibroblasts) as well as their proliferative response and their interaction were compared with those of other matrices. Results: Native fibrin glue does not release vascular endothelial growth factor ( VEGF) but is able to bind this growth factor in biologically relevant concentrations of 152.6 pg/mL. The addition of fibrin glue to a collagen type I matrix led to an increased proliferation rate of gastric wall fibroblasts. The transcription of VEGF and platelet-derived growth factor ( PDGF) mRNA was significantly increased in epithelial cells. Co-culture of fibroblasts grown on fibrin glue containing matrix and epithelial cells resulted in an increased secretion of VEGF by both cell lines. Conclusions: Fibrin glue leads to increased proliferation of fibroblasts and local accumulation of VEGF. These findings might at least partly explain the accelerated closure of bleeding ulcers treated by fibrin glue injection.