leafy hull sterile1 is a homeotic mutation in a rice MADS box gene affecting rice flower development
leafy hull sterile1 is a homeotic mutation in a rice MADS box gene affecting rice flower development
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DOI：
10.1105/tpc.12.6.871
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发表时间：
2000-06-01
期刊：
影响因子：
11.6
通讯作者：
An, G
中科院分区：
文献类型：
--
作者：
Jeon, JS;Jang, S;An, G
水稻含有多个 MADS 盒基因。先前已经证明，其中一个基因 OsMADS1（水稻 MADS 盒基因 1）优先在花中表达，当在烟草植物中异位表达时会导致提前开花。在这项研究中，我们证明了水稻中 OsMADS1 的异位表达也会导致提前开花。为了进一步研究 OsMADS1 在水稻花发育过程中的作用，我们生成了表达改变的 OsMADS1 基因的转基因水稻植物，这些基因在 MADS 结构域中包含错义突变。转基因植物在营养阶段没有明显的变化。然而，转基因圆锥花序通常表现出表型改变，包括由细长的叶状内稃和外稃组成的小穗，表现出开放壳的特征，两对叶状内稃和外稃状浆片，雄蕊数量减少，心皮数量增加。此外，一些小穗从同一小穗轴上产生了额外的小花。这些特征与叶壳不育1（lhs1）非常相似，OsMADS1的图谱位置与3号染色体上的lhs1紧密相连，lhs1的检查发现它在OsMADS1 MADS结构域中含有两个错义突变。遗传互补实验表明，含有野生型 OsMADS1 基因的 11.9-kb 基因组 DNA 片段挽救了突变表型，此外，从 lhs1 系中分离的 OsMADS1 基因的异位表达导致了 lhs1 赋予的表型，这些证据表明 OsMADS1 就是 lhs1 基因。
Rice contains several MADS box genes. It has been demonstrated previously that one of these genes, OsMADS1 (for Oryza sativa MADS box gene1), is expressed preferentially in flowers and causes early flowering when ectopically expressed in tobacco plants. In this study, we demonstrated that ectopic expression of OsMADS1 in rice also results in early flowering. To further investigate the role of OsMADS1 during rice flower development, we generated transgenic rice plants expressing altered OsMADS1 genes that contain missense mutations in the MADS domain. There was no visible alteration in the transgenic plants during the vegetative stage. However, transgenic panicles typically exhibited phenotypic alterations, including spikelets consisting of elongated leafy paleae and lemmas that exhibit a feature of open hull, two pairs of leafy palea-like and lemma-like lodicules, a decrease in stamen number, and an increase in the number of carpels. In addition, some spikelets generated an additional floret from the same rachilla. These characteristics are very similar to those of leafy hull sterile1 (lhs1), The map position of OsMADS1 is closely linked to that of lhs1 on chromosome 3, Examination of lhs1 revealed that it contains two missense mutations in the OsMADS1 MADS domain. A genetic complementation experiment showed that the 11.9-kb genomic DNA fragment containing the wild-type OsMADS1 gene rescued the mutant phenotypes, In addition, ectopic expression of the OsMADS1 gene isolated from the lhs1 line resulted in lhs1-conferred phenotypes, These lines of evidence demonstrate that OsMADS1 is the lhs1 gene.