In vivo MRI detection of atherosclerosis in ApoE-deficient mice by using tenascin-C-targeted USPIO
In vivo MRI detection of atherosclerosis in ApoE-deficient mice by using tenascin-C-targeted USPIO
复制标题
使用 Tenascin-C 靶向 USPIO 体内 MRI 检测 ApoE 缺陷小鼠的动脉粥样硬化
DOI：
10.1177/0284185118762613
复制
发表时间：
2018-12-01
期刊：
影响因子：
1.3
通讯作者：
Shi, Hai-bin
中科院分区：
文献类型：
--
作者：
Li, Yan;Liu, Jun;Shi, Hai-bin
背景：动脉粥样硬化是心脑血管疾病的主要病因。目的：探讨抗Tenascin-C-USPIO特异性探针作为7.0-T磁共振成像(MRI)检测动脉粥样硬化斑块的分子标志物的可能性。材料与方法：20只高脂饮食喂养的ApoE(-/-)小鼠分别于16周和24周用7.0-T MRI检测主动脉弓动脉粥样硬化斑块。靶组10只注射抗Tenascin-C-USPIO，对照组10只注射纯USPIO(每组n=5)。结果：靶向治疗组斑块相对信号强度(RSI)明显低于对照组(16周：-15.65+/-0.78%比-3.43+/-2.57%；24周：-26.38+/-1.54%比-11.12+/-1.60%；P<；0.05)。组织病理学分析显示，从16周到24周的时间内，可见动脉粥样硬化斑块的形成和发展。24周时斑块Tenascin-C表达高于16周时(0.22+/-0.04 vs.0.13+/-0.02，P<0.05)。结论：Tenascin-C的表达随着动脉粥样硬化的进展而增加。抗Tenascin-C-USPIO为MRI检测和监测动脉粥样硬化斑块提供了一种有用的分子成像工具。
Background: Atherosclerosis is the main cause of cardiovascular and cerebrovascular diseases. Non-invasive molecular imaging to detect and characterize the plaques is essential for reducing life-threatening cardiovascular events.Purpose: To investigate the possibility of the anti-tenascin-C-USPIO specific probe as a molecular marker of atherosclerotic plaques detected by 7.0-T magnetic resonance imaging (MRI).Material and Methods: Twenty ApoE(-/-) mice fed with a high fat diet were used for detecting the aorta arch atherosclerotic plaques by 7.0-T MRI at 16 and 24 weeks. Ten mice in the targeted group were injected with anti-tenascin-C-USPIO and another ten in the control group were injected with pure USPIO (n = 5 each time point in each group). Histopathologic examination was used to evaluate the plaques and immunohistochemistry analysis was used to compare tenascin-C expression.Results: The relative signal intensity (rSI) changes of the targeted group decreased more than those of the control group (16 weeks: -15.65 +/- 0.78% vs. -3.43 +/- 2.57%; 24 weeks: -26.38 +/- 1.54% vs. -11.12 +/- 1.60%, respectively; P < 0.05). Histopathological analyses demonstrated visible atherosclerotic plaques formation and development over time from 16 weeks to 24 weeks. Tenascin-C expression of the plaques at 24 weeks was higher than that at 16 weeks (0.22 +/- 0.04 vs. 0.13 +/- 0.02, P < 0.05). The MR images correlated well with the progression of atherosclerotic plaques.Conclusion: Tenascin-C expression increased with the progression of atherosclerosis. Anti-tenascin-C-USPIO could provide a useful molecular imaging tool for detecting and monitoring atherosclerotic plaques by MRI.