Serotype-specific invasiveness and colonization prevalence in Streptococcus pneumoniae correlate with the lag phase during in vitro growth
Serotype-specific invasiveness and colonization prevalence in Streptococcus pneumoniae correlate with the lag phase during in vitro growth
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DOI：
10.1016/j.micinf.2006.07.013
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发表时间：
2006-09-01
影响因子：
5.8
通讯作者：
Muehlemann, Kathrin
中科院分区：
文献类型：
--
作者：
Baettig, Patrick;Hathaway, Lucy J.;Muehlemann, Kathrin
肺炎链球菌的血清型在定植率和引起疾病的可能性方面有所不同。比较了代表 15 种肺炎球菌血清型的 47 种临床分离株在含有或不含 5% 胎牛血清 (FCS) 的脑心浸液肉汤中的体外生长情况。血清型特异性定植率和入侵潜力的比值比是从一项国际和当地流行病学研究中获得的。滞后期的持续时间随着侵袭性的增加而增加，并且与血清型的定植流行率呈负相关。补充 FCS 优先缩短与侵袭性疾病相关血清型的滞后期 (P = 0.007)。通过添加锰 (Mn2+)、钛试剂、甘露醇或过氧化氢酶来减少氧化应激不会显着影响滞后期的持续时间。肺炎链球菌的血清型特异性侵袭力和定植率与体外生长期间滞后期的长度相关。这可能与体内血清型特异性选择相关。 (c) 2006 年 Elsevier Masson SAS。版权所有。
Serotypes of Streptococcus pneumoniae differ in colonization prevalence and the likelihood of causing disease. In vitro growth in brain heart infusion broth with or without 5% fetal calf serum (FCS) was compared for 47 clinical isolates representing 15 pneumococcal serotypes. Serotype-specific colonization prevalence and odds ratios for the invasive potential were obtained from an international and a local epidemiological study. The duration of the lag phase increased with the invasiveness and was inversely associated with the colonization prevalence of a serotype. Supplementation with FCS shortened the lag phase preferentially in serotypes associated with invasive disease (P = 0.007). Reduction of oxidative stress by addition of manganese (Mn2+), Tiron, mannitol or catalase did not influence the duration of the lag phase significantly. Serotype specific invasiveness and colonization prevalence of S. pneumoniae are associated with the length of the lag phase during in vitro growth. This may correlate with serotype specific selection in vivo. (c) 2006 Elsevier Masson SAS. All rights reserved.