Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.
Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.
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DOI：
10.1038/nsmb.3145
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发表时间：
2016-01
影响因子：
16.8
通讯作者：
Gurdon JB
中科院分区：
文献类型：
--
作者：
Koziol MJ;Bradshaw CR;Allen GE;Costa ASH;Frezza C;Gurdon JB
胞嘧啶脱氧核苷酸（dC5m）的甲基化是一个公认的表观遗传标记，但在高等真核生物中，对影响其他脱氧核苷酸的修饰知之甚少。在这里，我们报告了脊椎动物DNA中n -6-甲基脱氧腺苷（dA6m）的检测，特别是非洲爪蟾，但也包括小鼠和人类在内的其他物种。我们的甲基组分析表明，dA6m广泛分布在真核生物基因组中，存在于不同的细胞类型中，但通常从基因外显子中消失。因此，直接的DNA修饰可能比以前认为的更为广泛。
Methylation of cytosine deoxynucleotides (dC5m) is a well-established epigenetic mark, but in higher eukaryotes much less is known about modifications affecting other deoxynucleotides. Here, we report the detection of N-6-methyl-deoxyadenosine (dA6m) in vertebrate DNA, specifically in Xenopus laevis, but also in other species including mouse and human. Our methylome analysis reveals that dA6m is widely distributed across the eukaryotic genome, is present in different cell types, but commonly depleted from gene exons. Thus, direct DNA modifications might be more widespread than previously thought.