Emergence of a multidrug resistance efflux pump with carbapenem resistance gene blaVIM-2 in a Pseudomonas putida megaplasmid of migratory bird origin
Emergence of a multidrug resistance efflux pump with carbapenem resistance gene blaVIM-2 in a Pseudomonas putida megaplasmid of migratory bird origin
复制标题
候鸟源恶臭假单胞菌巨质粒中具有碳青霉烯类耐药基因 blaVIM-2 的多药耐药外排泵的出现
DOI：
10.1093/jac/dkab044
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发表时间：
2021-03-24
影响因子：
5.2
通讯作者：
Wang, Zhiqiang
中科院分区：
文献类型：
--
作者：
Li, Ruichao;Peng, Kai;Wang, Zhiqiang
背景：替加环素和碳青霉烯类被认为是治疗严重细菌感染的重要抗菌药物。假单胞菌中同时存在替加环素和碳青霉烯类耐药基因。目的：目的：研究候鸟型恶臭假单胞菌（Pseudomonasputida）中共同携带tmexCD 1-toprJ 1和bla（Vim-2）基因的大质粒。方法：分离出一株对美罗培南和替加环素耐药的恶臭假单胞菌ZXPA-20，通过药敏试验、接合试验、WGS分析和生物信息学分析，对该菌进行鉴定。将基因bla（Vim-2）插入到大质粒pZXPA-20的骨架中的Tn 5090样结构内。megaplasm中tmexCD 1-toprJ 1的遗传背景与假单胞菌属的许多染色体tmexCD 1-toprJ 1相同。假单胞菌质粒介导的tmexCD 1-toprJ 1基因簇比肺炎克雷伯菌更常见。据我们所知，这是第一次报告的bla（Vim-2）和tmexCD 1-toprJ 1在一个plasmid.Conclusions：质粒介导的碳青霉烯类和替加环素耐药基因的出现在候鸟恶臭假单胞菌突出了新的移动的耐药基因在候鸟，这可能发挥了至关重要的作用，在全球传播的新的耐药基因监测的重要性。
Background: Tigecycline and carbapenems are regarded as vital antimicrobials to treat serious bacterial infections. Co-occurrence of resistance genes conferring resistance to both tigecycline and carbapenems in Pseudomonas spp. was not investigated.Objectives: To characterize a megaplasmid co-harbouring tmexCD1-toprJ1 and bla(VIM-2) in Pseudomonas putida of migratory bird origin.Methods: One tigecycline- and carbapenem-resistant strain was isolated and characterized by antimicrobial susceptibility testing, conjugation assay, WGS and bioinformatics analysis.Results: The strain P. putida ZXPA-20 resistant to meropenem and tigecycline was positive for bla(VIM-2) and tmexCD1-toprJ1 genes. The gene bla(VIM-2) was inserted into the backbone of the megaplasmid pZXPA-20 within a Tn5090-like structure. The genetic context of tmexCD1-toprJ1 in the megaplasmid was identical to many chromosomal tmexCD1-toprJ1 of Pseudomonas species. Plasmid-mediated tmexCD1-toprJ1 gene cluster in Pseudomonas spp. was more common than that in Klebsiella pneumoniae. To the best of our knowledge, this is the first report of co-occurrence of bla(VIM-2) and tmexCD1-toprJ1 in one plasmid.Conclusions: Emergence of plasmid-mediated carbapenem and tigecycline resistance genes in P. putida from migratory birds highlighted the importance of surveillance of novel mobile resistance genes in migratory birds, which may play a vital role in global transmission of novel resistance genes.