Effect of age on cognitive sequelae following early life seizures in rats
Effect of age on cognitive sequelae following early life seizures in rats
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DOI：
10.1016/j.eplepsyres.2009.03.008
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发表时间：
2009-08-01
影响因子：
2.2
通讯作者：
Holmes, Gregory L.
中科院分区：
文献类型：
--
作者：
Karnam, Havisha B.;Zhao, Qjan;Holmes, Gregory L.
目的：临床研究表明，新生儿癫痫发作比年龄较大的儿童癫痫发作更具破坏性。然而，这些研究很难解释各种因素，包括不同年龄癫痫发作的病因。动物研究可以提供洞察到的问题，是否在儿童癫痫发作的年龄是一个因素，在cognitive outcome.Methods：为了评估年龄对癫痫引起的认知功能障碍的影响，我们进行了大鼠50次癫痫发作从出生后的日子P0-P10或P15-P25。成年后，在Morris水迷宫、放射臂水迷宫、旷场和主动回避中研究大鼠。为了评估突触强度和网络的兴奋和抑制功能的动物进行了评价与tong-term potentiation（LTP）和成对脉冲促进/inhibitory.Results：与对照组相比，两组大鼠反复发作的空间记忆受损的水迷宫测试，有改变的活动在开放的领域，并没有显着差异，从控制在主动回避。反复发作的大鼠LTP受损，但在成对脉冲易化或抑制方面没有表现出缺陷。虽然发病较晚的大鼠表现出更差的性能比早期癫痫发作的大鼠的趋势，差异并不substantial.Conclusions：在发展过程中反复发作与学习，记忆和活动水平，以及受损的突触效率与tong-term行为缺陷。癫痫发作的年龄不是一个强有力的预测结果。(C)2009 Elsevier B. V.保留所有权利。
Purpose: Clinical studies have suggested that seizures in newborns are more damaging than seizures occurring in older children. However, these studies are difficult to interpret for a variety of factors including differing etiologies of seizures across ages. Animal studies can provide insights into the question of whether age of seizure onset in children is a factor in cognitive outcome.Methods: To evaluate the effect of age on seizure-induced cognitive impairment we subjected rats to 50 seizures from postnatal days P0-P10 or P15-P25. As adults the rats were studied in the Morris water maze, radial-arm water maze, open field, and active avoidance. To assess synaptic strength and network excitatory and inhibitory function animals were evaluated with tong-term potentiation (LTP) and paired-pulse facilitation/inhibition.Results: Compared to controls, both groups of rats with recurrent seizures were impaired in spatial memory in both water maze tests, had altered activity in the open field, and did not differ from controls in active avoidance. Rats with recurrent seizures had impaired LTP but showed no deficits in paired-pulse facilitation or inhibition. While rats with later onset showed a trend to worse performance than rats with earlier seizures, the differences were not substantial.Conclusions: Recurrent seizures during development are associated with tong-term behavioral deficits in learning, memory and activity level as well as impaired synaptic efficiency. Age of seizure onset was not a strong predictor of outcome. (C) 2009 Elsevier B.V. All rights reserved.