3D culture of neural stem cells within conductive PEDOT layer-assembled chitosan/gelatin scaffolds for neural tissue engineering
3D culture of neural stem cells within conductive PEDOT layer-assembled chitosan/gelatin scaffolds for neural tissue engineering
复制标题
用于神经组织工程的导电 PEDOT 层组装壳聚糖/明胶支架内神经干细胞的 3D 培养
DOI：
10.1016/j.msec.2018.08.054
复制
发表时间：
2018-12-01
影响因子：
7.9
通讯作者：
Ma, Xuehu
中科院分区：
文献类型：
--
作者：
Wang, Shuping;Guan, Shui;Ma, Xuehu
神经干细胞（NSC）作为一种自我更新和多能细胞群，因其不可再生而被广泛研究。工程支架是调节NSCs增殖和分化以形成所需细胞和组织的重要因素之一。由于神经细胞是电活性细胞，因此需要导电支架来提供三维细胞生长微环境和适当的协同细胞引导线索。本研究通过原位界面聚合制备了聚(3,4-乙撑二氧噻吩)/壳聚糖/明胶(PEDOT/Cs/Gel)支架，并在多孔Cs/Gel支架的通道表面组装了纳米结构的PEDOT层。这种导电、三维、多孔且可生物降解的 PEDOT/Cs/Gel 支架被用作 NSC 体外三维 (3D) 培养的新型支架。研究发现Cs/Gel支架通道表面的PEDOT层可以极大地促进NSCs的粘附和增殖。此外，在分化条件下，蛋白质和基因分析表明PEDOT/Cs/Gel支架可以显着增强NSCs向神经元和星形胶质细胞的分化，并上调β微管蛋白-III和GFAP的表达。总之，这些结果表明，PEDOT/Cs/Gel支架作为导电支架不仅可以促进NSCs粘附和增殖，而且可以增强NSCs分化为具有更高蛋白质和基因表达的神经元和星形胶质细胞。 PEDOT 组装的 Cs/Gel 支架将成为 NSC 研究和神经组织工程的一种有前途的导电基质。
Neural stem cells (NSCs), as a self-renewing and multipotent cell population, have been widely studied for never regeneration. Engineering scaffold is one of the important factors to regulate NSCs proliferation and differentiation towards the formation of the desired cells and tissues. Because neural cells are electro-active ones, a conductive scaffold is required to provide three-dimensional cell growth microenvironments and appropriate synergistic cell guidance cues. In this study, a poly (3,4-ethylenedioxythiophene)/chitosan/gelatin (PEDOT/Cs/Gel) scaffold was prepared via in situ interfacial polymerization, with a nanostructured layer of PEDOT assembling on the channel surface of porous Cs/Gel scaffold. This electrically conductive, three-dimensional, porous and biodegradable PEDOT/Cs/Gel scaffold was used as a novel scaffold for NSCs three-dimension (3D) culture in vitro. It was found that the layer of PEDOT on the channel surface of Cs/Gel scaffolds could greatly promote NSCs adhesion and proliferation. Additionally, under the differentiation condition, the protein and gene analysis suggested that PEDOT/Cs/Gel scaffolds could significantly enhance the NSCs differentiation towards neurons and astrocytes with the up-regulation of beta tubulin-III and GFAP expression. In conclusion, these results demonstrated that the PEDOT/Cs/Gel scaffolds as an electrically conductive scaffold could not only promote NSCs adhesion and proliferation but also enhance NSCs differentiation into neurons and astrocytes with higher protein and gene expression. PEDOT-assembled Cs/Gel scaffold will be a promising conductive substrate for NSCs research and neural tissue engineering.