Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
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DOI：
10.1007/978-1-59745-019-5_18
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发表时间：
2010-01-01
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影响因子：
--
通讯作者：
Kokaia, Zaal
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文献类型：
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作者：
Ahlenius, Henrik;Kokaia, Zaal
神经干细胞是由中枢神经系统细胞衍生而来的细胞，具有干细胞特有的特性，即自我更新和多能性。在培养条件下扩增神经干细胞的一种广泛使用的方法是基于这些细胞在添加各种生长因子的无血清培养基中培养时连续分裂的能力。一种常用的方法是将神经干细胞培养成自由漂浮的细胞聚集体，称为神经球。神经球可以从胚胎和成年哺乳动物大脑的几种结构中产生。虽然可以从大脑的粗提取物中产生可存活的细胞系，但精确的解剖是获得纯细胞群的关键。在这里，我们描述了从小鼠和大鼠的胚胎神经节突起和成年室下带分离、分离和生成神经球的方法。
Neural stem cells are defined as cells that either gives rise to or derives from the cells of the central nervous system and have the unique properties of stem cells, i.e. self-renewal and multipotentiality. One of the widely used methods of expanding neural stem cells under culture conditions is based on the capacity of these cells to divide continuously when cultured in serum-free medium supplemented with various growth factors. One common method used is to grow neural stem cells as free-floating aggregates of cells called neurospheres. Neurospheres can be generated from several structures of the embryonic and adult mammalian brain. Although viable lines can be generated from crude extracts of brain, a precise dissection is crucial to get a pure population of cells. Here we describe methods for dissection, isolation and generation of neurospheres from embryonic ganglionic eminences and adult subventricular zone of mice and rats.