Duplication polymorphism within the third intron of the p53 gene is a rare event in Japanese population
Duplication polymorphism within the third intron of the p53 gene is a rare event in Japanese population
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p53 基因第三个内含子内的重复多态性在日本人群中是罕见的事件
DOI：
10.1007/bf01915955
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
M. Yamakido
中科院分区：
文献类型：
--
作者：
K. Hiyama;Masahiro Takeda;Y. Shirotani;S. Ishioka;Y. Awaya;K. Inai;K. Inyaku;S. Hozawa;T. Inamizu;M. Yamakido
材料和方法我们从183名日本人（包括56名肺癌患者）的外周血白细胞或非癌肺组织中获得基因组DNA。设计PCR引物，扩增出p53基因第3内含子上460 bp（A1）和476 bp（A2）的多态性重复序列。将基因组DNA（100 ng）在含有200 pM每种dNTP、1.5 mM MgCl-、0.25 pM每种引物（正义和反义引物：5 '-GGACTGACTTTCTGCTCTTG-3'，5 '-TGAAGTCTCATGGAAGCC-3'）的50 μ 1反应混合物中扩增。
Materials and MethodsWe obtained genomic DNA from peripheral leucocytes or non-cancerous lung tissues from 183 unrelated Japanese individuals including 56 lung cancer patients. PCR primers were designed to amplify a 460 bp (A1) and 476 bp (A2) containing the polymorphic repeat on the third intron of the p53 gene. Genomic DNA (100 ng) was amplified in a 50 t~ l reaction mixture containing 200 pM each dNTP, 1.5 mM MgCI~, 0.25 pM each primer (sense and antisense primers: 5'-GGACTGACTTTCTGCTCTTG-3', 5'-TGAAGTCTCATGGAAGCC-