Myeloid and megakaryocytic properties of K-562 cell lines.
Myeloid and megakaryocytic properties of K-562 cell lines.
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发表时间：
1983-10
期刊：
影响因子：
11.2
通讯作者：
Antonio Tabilio;P. Pelicci;G. Vinci;P. Mannoni;Curt I. Civin;W. Vainchenker;Ugo Testa;M. Lipiński-M
中科院分区：
文献类型：
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作者：
Antonio Tabilio;P. Pelicci;G. Vinci;P. Mannoni;Curt I. Civin;W. Vainchenker;Ugo Testa;M. Lipiński-M
髓系和巨核细胞分化标记物在一个K-562细胞亚系、其克隆和原始细胞系中的表达已被研究。用氯化高铁血红素、丁酸钠和12-O-十四烷基佛波醇-13-乙酸酯诱导前后的K-562细胞进行细胞毒性、电子显微镜、一组多克隆抗体和单抗的免疫荧光检测以及放射免疫分析。所有K-562细胞系均表达髓系膜标志物。75%~95%的细胞仅表达早期粒系细胞表面标志，未检测到晚期膜标志。相反，早期(髓过氧化物酶)和晚期(乳铁蛋白)细胞质标记物都不存在。因此，K-562细胞表现出与正常或白血病早幼粒细胞相似的膜表型，但缺乏髓过氧化物酶。在K-562细胞中还检测到了膜巨核细胞标志物，如血小板膜糖蛋白IIIa和血小板过氧化物酶。然而，其他一些早期巨核细胞标志物，如血小板膜糖蛋白1b、凝血因子VIII-R-Ag和血小板膜因子4，用荧光标记无法检测到。克隆该细胞系并未导致选择一个单潜能细胞系。这些结果可以用单个细胞中多系标记的表达来解释。在所有的细胞系和克隆中，氯化血红素略微增加了髓系膜标志物的表达，而不改变巨核细胞标志物。丁酸钠和12-O-十四酰佛波醇-13-乙酸酯降低了大多数髓系标志物，显著增加了巨核细胞标志物的表达。
The expression of myeloid and megakaryocytic markers of differentiation has been studied in one K-562 cell subline, in its clones, and in the original cell line. Cytotoxicity, electron microscopy, immunofluorescence studies with a panel of polyclonal and monoclonal antibodies, and radioimmunoassays were performed on K-562 cells before and after induction with hemin, sodium butyrate, and 12-O-tetradecanoylphorbol-13-acetate. Myeloid membrane markers were present in all K-562 cell lines. Only the early granulopoietic cell surface markers were expressed in 75 to 95% of the cells, while none of the late membrane markers was detected. In contrast, neither the early (myeloperoxidase) nor late (lactoferrin) cytoplasmic markers were present. Thus, K-562 cells showed a membrane phenotype similar to that of a normal or leukemic promyelocyte but lacking myeloperoxidase. Membrane megakaryocytic markers, such as platelet glycoprotein IIIa and platelet peroxidase, were also detected in K-562 cells. However, some other early megakaryocytic markers, such as platelet glycoprotein lb, Factor VIII-R-Ag, and platelet Factor 4, could not be detected by fluorescent labeling. Cloning of the cell line did not result in the selection of a unipotential cell line. These results could be explained by the expression of multilineage markers in a single cell. In all of the cell lines and clones, hemin slightly increased the expression of the myeloid membrane markers without any modification of the megakaryocytic markers. Sodium butyrate and 12-O-tetradecanoylphorbol-13-acetate diminished most of the myeloid markers and very significantly increased the expression of the megakaryocytic markers.