Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice
Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice
复制标题
DOI：
10.1007/s00122-015-2502-4
复制
发表时间：
2015-07-01
影响因子：
5.4
通讯作者：
Singh, Ashok K.
中科院分区：
文献类型：
--
作者：
Khanna, Apurva;Sharma, Vinay;Singh, Ashok K.
已开发出一组在印度香米品种中携带主要稻瘟病抗性基因的 NIL。此外，金字塔相对于单基因 NIL 对抗稻瘟病菌 Magnaporthe oryzae 的功效已得到证实。印度香米的生产力和质量受到稻瘟病的严重影响。仅在非印度香米种质中报道了赋予稻瘟病抗性的主要基因和 QTL。在这里，我们报道了来自供体系 DHMASQ164-2a (Pi54、Pi1、Pita)、IRBLz5-CA (Pi2)、IRBLb-B (Pib)、IRBL5-M (Pi5) 和 IRBL9-W (Pi9) 的七个稻瘟病抗性基因整合到优良巴斯马蒂水稻品种 Pusa Basmati 1 (PB1) 的遗传背景中。通过标记辅助回交育种 (MABB) 开发了总共 36 个近等基因系 (NIL)，其中包括 14 个单基因金字塔、16 个二基因金字塔和 6 个三基因金字塔。在 NIL 中，前景、重组和背景选择分别用于识别具有目标基因的植物、最小化连锁阻力并增加轮回亲本基因组 (RPG) 回收率 (93.5-98.6%)。使用 50,051 个 SNP 和 500 个 SSR 标记进行的比较分析表明，SNP 可以更好地了解 RPG 恢复情况。大多数单基因 NIL 在产量和质量方面表现出相当的性能，同时，与轮回亲本 (RP) PB1 相比，Pusa1637-18-7-6-20 (Pi9) 在产量上显着优于并且在四种不同环境中保持稳定。此外，在金字塔中，与RP PB1相比，Pusa1930-12-6（Pi2+Pi5）的出品率显着更高，Pusa1633-7-8-53-6-8（Pi54+Pi1+Pita）的烹饪品质优异。携带 Pi9 基因的 NIL 被发现对对抗稻瘟病热点地区主要的有毒菌种的混合物最有效。相反，当在人工接种下进行分析时，与两基因和单基因 NIL 相比，三基因金字塔表现出增强的抗性。
A set of NILs carrying major blast resistance genes in a Basmati rice variety has been developed. Also, the efficacy of pyramids over monogenic NILs against rice blast pathogen Magnaporthe oryzae has been demonstrated.Productivity and quality of Basmati rice is severely affected by rice blast disease. Major genes and QTLs conferring resistance to blast have been reported only in non-Basmati rice germplasm. Here, we report incorporation of seven blast resistance genes from the donor lines DHMASQ164-2a (Pi54, Pi1, Pita), IRBLz5-CA (Pi2), IRBLb-B (Pib), IRBL5-M (Pi5) and IRBL9-W (Pi9) into the genetic background of an elite Basmati rice variety Pusa Basmati 1 (PB1). A total of 36 near-isogenic lines (NILs) comprising of 14 monogenic, 16 two-gene pyramids and six three-gene pyramids were developed through marker-assisted backcross breeding (MABB). Foreground, recombinant and background selection was used to identify the plants with target gene(s), minimize the linkage drag and increase the recurrent parent genome (RPG) recovery (93.5-98.6 %), respectively, in the NILs. Comparative analysis performed using 50,051 SNPs and 500 SSR markers revealed that the SNPs provided better insight into the RPG recovery. Most of the monogenic NILs showed comparable performance in yield and quality, concomitantly, Pusa1637-18-7-6-20 (Pi9), was significantly superior in yield and stable across four different environments as compared to recurrent parent (RP) PB1. Further, among the pyramids, Pusa1930-12-6 (Pi2+Pi5) showed significantly higher yield and Pusa1633-7-8-53-6-8 (Pi54+Pi1+Pita) was superior in cooking quality as compared to RP PB1. The NILs carrying gene Pi9 were found to be the most effective against the concoction of virulent races predominant in the hotspot locations for blast disease. Conversely, when analyzed under artificial inoculation, three-gene pyramids expressed enhanced resistance as compared to the two-gene and monogenic NILs.