CONFORMATIONAL-CHANGES IN PROTEINS PROBED BY HYDROGEN-EXCHANGE ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY
CONFORMATIONAL-CHANGES IN PROTEINS PROBED BY HYDROGEN-EXCHANGE ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY
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DOI：
10.1002/rcm.1290050415
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发表时间：
1991-04-01
影响因子：
2
通讯作者：
CHAIT, BT
中科院分区：
文献类型：
--
作者：
KATTA, V;CHAIT, BT
氢交换电喷雾电离质谱法是一种有效的探测溶液中蛋白质构象变化的新方法。该方法是基于在规定的时间内不同蛋白质构象中发生的氢/氘交换程度的质谱测量。结果提出了氢交换电喷雾电离质谱法探测的构象变化的牛泛素蛋白的酸性水溶液中加入甲醇诱导。
Hydrogen-exchange electrospray-ionization mass spectrometry is demonstrated to be an effective new method for probing conformational changes of proteins in solutions. The method is based on the mass spectrometric measurement of the extent of hydrogen/deuterium exchange that occurs in different protein conformers over defined periods of time. Results are presented in which hydrogen-exchange electrospray-ionization mass spectrometry is used to probe conformational changes in bovine ubiquitin induced by the addition of methanol to aqueous acidic solutions of the protein.