Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots
Subdiffraction fluorescence imaging of biomolecular structure and distributions with quantum dots
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DOI：
10.1016/j.bbamcr.2010.06.004
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发表时间：
2010-10-01
影响因子：
5.1
通讯作者：
Heilemann, Mike
中科院分区：
文献类型：
--
作者：
Heidbreder, Meike;Endesfelder, Ulrike;Heilemann, Mike
我们引入了基于半导体量子点的荧光成像，在三维空间中具有类似于2倍的光学分辨率，作为一种方法，可以研究细胞结构和膜和亚细胞器中生物分子的空间组织。通过免疫细胞化学用量子点标记靶生物分子。通过量子点的三光子吸收和随后的高阶激子态的荧光发射来实现分辨率增强。与传统的多光子显微镜不同，这种方法可以在任何共焦显微镜上实现，而不需要脉冲激发光。我们展示了U373细胞微管网络的量子点三激子成像（QDTI），HeLa细胞质膜上TNF受体2的3D成像，以及COS-7细胞中线粒体细胞色素c氧化酶和肌动蛋白的3D成像。(C)2010爱思唯尔有限公司版权所有。
We introduce semiconductor quantum dot-based fluorescence imaging with similar to 2-fold increased optical resolution in three dimensions as a method that allows both studying cellular structures and spatial organization of biomolecules in membranes and subcellular organelles. Target biomolecules are labelled with quantum dots via immunocytochemistry. The resolution enhancement is achieved by three-photon absorption of quantum dots and subsequent fluorescence emission from a higher-order excitonic state. Different from conventional multiphoton microscopy, this approach can be realized on any confocal microscope without the need for pulsed excitation light. We demonstrate quantum dot triexciton imaging (QDTI) of the microtubule network of U373 cells, 3D imaging of TNF receptor 2 on the plasma membrane of HeLa cells, and multicolor 3D imaging of mitochondrial cytochrome c oxidase and actin in COS-7 cells. (C) 2010 Elsevier B.V. All rights reserved.