The effect of sodium chloride and sodium bicarbonate derived anolytes, and anolyte-catholyte combination on biofilms
The effect of sodium chloride and sodium bicarbonate derived anolytes, and anolyte-catholyte combination on biofilms
复制标题
氯化钠和碳酸氢钠衍生的阳极电解液以及阳极电解液-阴极电解液组合对生物膜的影响
DOI：
10.4314/wsa.v32i2.5248
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发表时间：
2007
期刊：
影响因子：
1.5
通讯作者：
T. E. Cloete
中科院分区：
文献类型：
--
作者：
M. Thantsha;T. E. Cloete
在大面积的水下表面暴露于环境中的工业环境中，微生物生物膜是一个问题
相对较高的养分通量，为大量表面相关生长的形成提供了利基。饮用水分配管道中生长的生物膜会导致水的微生物质量恶化，从而导致水传播疾病的发生。许多细菌对中等水平的杀菌剂具有抗药性，其中生物膜中的细菌是最具抵抗力的。
难以控制。电化学活化（ECA）技术提供了另一种控制这些微生物的方法。本研究的主要目的是评估电化学活化溶液阳极电解液作为
用于控制生物膜的环境安全消毒剂。使用 Pederson 装置生长生物膜，然后暴露于不同浓度的杀菌剂。使用光学显微镜和扫描电子显微镜观察处理对生物膜结构的影响。通过阳极电解液处理后生物膜的重新生长通过
DAPI 染色后的落射荧光显微镜检查。纯净（未稀释）和轻度稀释的阳极电解液去除了
生物膜，而更稀的阳极电解液对生物膜没有任何影响。生物膜重新生长后发生
用阳极电解液和阳极电解液-阴极电解液组合进行生物膜处理24小时，通过菌落形成单位的增加来显示。
然而，浮游细菌仅在处理 72 小时后才重新生长。水 SA 卷。 32(2) 2006：第 237-242 页
Microbial biofilms are problematic in industrial environments where large areas of submerged surfaces are exposed to
relatively high nutrient fluxes, providing niches for the formation of copious surface-associated growth. Biofilms growing in drinking water distribution pipes cause deterioration in the microbiological quality of water contributing to the occurrence of water-borne diseases. Many bacteria are resistant to moderate levels of biocides, with bacteria in biofilms being the most
difficult to control. Electrochemical activation (ECA) technology provides an alternative way of controlling these micro-organisms. The main objective of this study was to evaluate an electrochemically activated solution, anolyte, as an
environmentally safe disinfectant for the control of biofilms. Biofilms were grown using the Pederson device and then exposed to different concentrations of the biocide. Light microscopy and scanning electron microscopy were used to view the effect of treatment on the biofilm structure. Re-growth of the biofilm after treatment with anolyte was detected through
epifluorescence microscopy after DAPI staining of the coupons. Neat (undiluted) and mildly dilute anolyte removed the
biofilm while the more dilute anolyte did not have any effect on the biofilm. Re-growth of the biofilm occurred after
24 h of biofilm treatment with anolyte and anolyte-catholyte combination, showed by the increase in colony forming units.
Re-growth of planktonic bacteria however, occurred only after 72 h of treatment. Water SA Vol. 32(2) 2006: pp.237-242