DISTRIBUTION AND REGULATION OF AROMATASE-ACTIVITY IN THE RAT HYPOTHALAMUS AND LIMBIC SYSTEM
DISTRIBUTION AND REGULATION OF AROMATASE-ACTIVITY IN THE RAT HYPOTHALAMUS AND LIMBIC SYSTEM
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DOI：
10.1210/endo-117-6-2471
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发表时间：
1985-01-01
期刊：
影响因子：
4.8
通讯作者：
RESKO, JA
中科院分区：
文献类型：
--
作者：
ROSELLI, CE;HORTON, LE;RESKO, JA
在大鼠大脑中雄激素转化为雌激素是由芳香化酶催化的。这些酶的最高浓度在下丘脑和杏仁核中发现，它们似乎在雄激素影响行为和神经内分泌功能的过程中起着重要作用。在本研究中，我们测量了成年大鼠脑20个核和脑区的芳香化酶活性（AA）水平。单个细胞核在600- 0.8 μ范围内进行显微解剖。M冷冻切片。收集3只动物的组织，通过体外放射测定法测定AA，该方法量化了[1. β]中3H2O的立体特异性产生。-3H]雄烯二酮作为雌激素形成的指标。我们报道AA在大鼠脑内的分布是不均匀的。活性最高的是终纹床核（n.）（700蛋白fmol/h .cntdot）。内侧（MA）和皮质杏仁核（400-600 fmol/h）。毫克蛋白质)的雄性。在整个间脑中，AA有明显的背侧-尾侧和中外侧梯度。脑室周围视前神经和内侧视前神经活性高；中间分布于视交叉上视前神经、下丘脑前部、下丘脑脑室周围前部和腹内侧神经；弓形中隆起较低，外侧视前隆起，视上隆起，背内侧隆起和外侧下丘脑。缺乏可测量AA的区域包括内侧和外侧隔膜、尾状壳核、海马和顶叶皮层。在雌性中，AA在MA和杏仁核皮层中最大。我们发现MA，终纹n.，视交叉上n.，脑室周围n.。其中，视前内侧n.，下丘脑前部n.，腹内侧n.，男性显著高于女性（P < 0.05）。睾丸切除术将AA降低到雌性的水平，而阉割雄性的睾丸激素则恢复了这些区域的AA。在其他下丘脑或杏仁核中没有观察到明显的性别差异，尽管在脑室周围下丘脑前、弓状中位隆起和外侧下丘脑，睾酮治疗增加了AA。我们的结果提供了AA在大鼠大脑特定下丘脑和边缘核的定量特征，以及在这些离散区域内AA控制的信息。
Conversion of androgen to estrogen in the rat brain is catalyzed by aromatase enzyes. The maximum concentrations of these enzymes are found within the hypothalamus and amygdala, where they appear to play an important role in the process by which androgens affect both behavior and neuroendocrine function. In the present study, we measured the levels of aromatase activity (AA) in 20 nuclei and brain regions of the adult rat brain. Individual nuclei were microdissected from 600-.mu.m frozen sections. Tissues from 3 animals were pooled, and AA was measured by an in vitro radiometric assay that quantifies the stereospecific production of 3H2O from [1.beta.-3H]androstenedione as an index of estrogen formation. We report that AA is heterogeneously distributed within the rat brain. The greatest amounts of activity were found in the bed nucleus (n.) of the stria terminalis (700 protein fmol/h .cntdot. mg) and in the medial (MA) and cortical amygdala (400-600 fmol/h .cntdot. mg protein) of the male. There was an evident rostal-caudal and medial-lateral gradient in AA throughtout the diencephalon. Activity was high in the periventricular preoptic n. and medial preoptic n.; intermediate in the suprachiasmatic preoptic n., anterior hypothalamus, periventricular anterior hypothalamus, and ventromedial n.; and low in the arcuate n.-medium eminence, lateral preoptic n., supraoptic n., dorsomedial n., and lateral hypothalamus. Regions devoid of measurable AA included the medial and lateral septum, caudate-putamen, hippocampus, and parietal cortex. In the female, AA was greatest in the MA and cortical amygdala. We found that AA in the MA, stria terminalis n., suprachiasmatic preoptic n., periventricular preoptic in., medial preoptic n., anterior hypothalamus, and ventromedial n. was significantly greater (P < 0.05) in males than in females. Orchidectomy reduced AA to levels seen in females, and administration of testosterone to castrated males restored AA in these areas. No significant sex differences were observed in any other hypothalamic or amygdaloid nuclei, although AA was increased by testosterone treatment in the periventricular anterior hypothalamus, arcuate n.-median eminence, and lateral hypthalamus. Our results provide a quantiatitve profile of AA in specific hypothalamic and limbic nuclei of the rat brain as well as information on the control of AA within these discrete regions.