rab3-Peptide stimulates exocytosis of the ram sperm acrosome via interaction with cyclic AMP and phospholipase A(2) metabolites
rab3-Peptide stimulates exocytosis of the ram sperm acrosome via interaction with cyclic AMP and phospholipase A(2) metabolites
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DOI：
10.1016/0014-5793(96)00749-1
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发表时间：
1996-08-12
期刊：
影响因子：
3.5
通讯作者：
Roldan, ERS
中科院分区：
文献类型：
--
作者：
Garde, J;Roldan, ERS
rab 3AL能增强A23187/Ca ~（2+）触发的顶体胞吐作用。rab 3AL的作用不在于精子的胞吐作用，也不在于其上游，磷脂酶A（2）（PLA（2）），当它与溶血磷脂酰胆碱一起包含时，rab 3AL克服了PLA（2）抑制剂马兜铃酸对胞吐的抑制; H-89阻止了这种作用。这些结果表明rab 3蛋白、PLA产生的代谢物（2）和cAMP激活的PKA之间存在功能性偶联，在顶体胞吐过程中导致膜融合的最后步骤。
Acrosomal exocytosis triggered with A23187/Ca2+ was enhanced by rab3AL, a synthetic peptide corresponding to the effector domain of the small GTP-binding protein rab3, Exocytosis was further enhanced when spermatozoa were also exposed to dibutyryl-cAMP, but was prevented when H-89, a protein kinase A (PKA) inhibitor, was included, The action of rab3AL was not on, or upstream of, phospholipase A(2) (PLA(2)), Inhibition of exocytosis by the PLA(2) inhibitor aristolochic acid was overcome by rab3AL when it was included together with lysophosphatidylcholine; this effect was prevented by H-89, These results suggest a functional coupling between rab3 protein, metabolites generated by PLA(2), and cAMP-activated PKA, in the final steps leading to membrane fusion during acrosomal exocytosis.